Molecular detection and controlling of seed-borne colletotrichum spp. in common bean and soybean
Other Title(s)
الكشف الجزيئي و مكافحة أنواع الفطر .Colletotrichum spp المحمول ببذور الفاصوليا و فول الصوديا
Joint Authors
Zayid, Muhammad Amin Abd al-Munim
Atiyyah, Mahmud Muhammad Muhammad
Ghunaym, Majdi Ibrahim
Suwaydi, Mahmud al-Sayyid
Source
Zagazig Journal for Agricultural Researches
Issue
Vol. 46, Issue 6 (31 Dec. 2019)17 p.
Publisher
Zagazig University Faculty of Agriculture
Publication Date
2019-12-31
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Topics
- Soybean
- Beans
- Macrophomina phaseolina
- Plant extracts
- Green bean
- Polymerase chain reaction
- Aspergillus flavus
- Rhizoctonia solani
- Aspergillus niger
- Fusarium solani
- Alternaria alternata
- Bacillus subtilis
Abstract EN
Seed-borne fungi cause several diseases of common bean (Phaseolus vulgaris L.
) and soybean (Glycine max L.
).
The survey of seed-borne fungi of common bean and soybean was carried out on three Egyptian Governorates (Behera, Dakahlia, and Ismailia) in Egypt.
Nineteen fungal species comprising therteen genera were isolated from the collected common bean and soybean seed samples, using standard blotter i, e.
Alternaria alternata, Alternaria spp.
, Aspergillus niger, Aspergillus ochraceous, Aspergillus flavus, Botryodiplodia sp.
, Cladosporium sp.
, Colletotrichum lindemuthianum, Colletotrichum dematium, Fusarium solani, Fusarium moniliforme, Fusarum oxysporum, Macrophomina phaseolina, Myrothecium sp.
, Penicillium spp, Rhizoctonia solani, Stemphylium spp.
, Trichoderma spp.
and Trichothecium sp.
Pathogenicity tests proved that C.
lindemuthianum and C.
dematium were pathogenic to common bean and soybean.
Polymerase chain reaction (PCR) has many beneficial characteristics that make it highly applicable for detecting Colletotrichum spp.
of seeds.
PCR diagnosis method and DNA extraction considered one of the most important steps and purity of DNA template for successful PCR assay.
For the PCR amplification of C.
lindemuthianum and C.
dematium, two primers ClF4 and ClF5, stander blotter, agar plate, and deep freezing method were used as seed healthy testing methods.
Stander blotter was proved the past and quickly method to detect seed-born of C.
lindemuthianum and C.
dematium.
Trichoderma harzianum reduced linear growth for C.
lindemuthianum and C.
dematium followed by Trichoderma viride and Bacillus subtilis recording (3.3 cm and 3.9 cm, respectively) followed by Pseudomonas floursence that display a high linear growth (7.4 cm).
On the other hand, fungicide Aetro 30% (Iprodione + Tebuconazole) recorded the least linear growth (0.9 cm) for C.
lindemuthianum and C.
dematium compared with negative control recorded (9.0 cm in diameter).
Thymus plant extract caused the lowest liner growth (3.7 cm).
Plant extract concentrations caused a significant reduction in the growth of C.
lindemuthianum and C.
dematium.
The highest concentration (1.5 % ) gave less fungal growth while the lowest one (0.5% ) showed the highest fungal growth compared with negative control.
American Psychological Association (APA)
Suwaydi, Mahmud al-Sayyid& Atiyyah, Mahmud Muhammad Muhammad& Zayid, Muhammad Amin Abd al-Munim& Ghunaym, Majdi Ibrahim. 2019. Molecular detection and controlling of seed-borne colletotrichum spp. in common bean and soybean. Zagazig Journal for Agricultural Researches،Vol. 46, no. 6.
https://search.emarefa.net/detail/BIM-1021775
Modern Language Association (MLA)
Suwaydi, Mahmud al-Sayyid…[et al.]. Molecular detection and controlling of seed-borne colletotrichum spp. in common bean and soybean. Zagazig Journal for Agricultural Researches Vol. 46, no. 6 (2019).
https://search.emarefa.net/detail/BIM-1021775
American Medical Association (AMA)
Suwaydi, Mahmud al-Sayyid& Atiyyah, Mahmud Muhammad Muhammad& Zayid, Muhammad Amin Abd al-Munim& Ghunaym, Majdi Ibrahim. Molecular detection and controlling of seed-borne colletotrichum spp. in common bean and soybean. Zagazig Journal for Agricultural Researches. 2019. Vol. 46, no. 6.
https://search.emarefa.net/detail/BIM-1021775
Data Type
Journal Articles
Language
English
Notes
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Record ID
BIM-1021775