Propagation of some tomato hybrids via tissue culture technique

Abstract EN

This investigation was carried out at the Tissue Culture Lab.

, Hort.

Dept.

, Fac.

Agric.

, Zagazig Univ.

, Egypt during the two successive seasons of 2014 and 2015 to producetransplants of two tomato hybrids through tissue culture technique.

A protocol was developed forshoot multiplication and rooting in two tomato (Lycopersicon esculentum Mill.

) hybrids i.

e.

, Silla 108and Farrah.

Shoot tips were cultured into Murashige and Skoog media (MS medium) with differentconcentrations of benzyl adenine (BA) and Kinetin (Kin) 1.5 mg BA/l, 1.5 mg BA/l + 1 mg Kinetin/l, 3 mg Kin/l and 4 mg Kin/l) compared with MS basal medium as a control treatment, the obtainedresults illustrated that supplementing BA at 1.5 mg/l gave the highest value of number ofshoots/explant of Sila 108 hybrid followed by kinetin at 4 mg/l for Farrah hybrid in both seasons.

Multiplicated shoots of two tomato hybrids were excised and cultured on MS medium supplementedwith different rooting growth regulators including indole acetic acid (IAA) at 0.5 and 1 mg/l, Naphthalene acetic acid (NAA) at 1 mg/l and indole butyric acid (IBA) at 1mg/l compared to thecontrol treatment (without growth regulators).

From the foregoing results, it could be concluded thatsupplementing IAA at 1 mg/l and NAA at 1 mg/l to MS media were the best treatment for rootingstage of Farrah and Sila 108 tomato hybrids.

The plantlets were transferred to plastic cups containingpeatmoss + sand at 1: 1, peatmoos + vermiculite at 1: 1 (V/V) or peatmoss alone.

It could be concludedthat the agriculture media for acclimatization of plantlets of Farrah and Sila 108 tomato hybridsproduced from tissue culture technique were peatmoss and peatmoss+ vermiculite at 1: 1 (V/V).