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Herpesvirus-Mediated Delivery of a Genetically Encoded Fluorescent Ca2+ Sensor to Canine Cardiomyocytes
Joint Authors
Papp, Julius G.
Jost, Norbert
Tóth, Judit S.
Varró, András
Virág, László
Boldogkői, Zsolt
Kristóf, Attila A.
Ördög, Balázs
Kovács, Péter P.
Nagy, Norbert
Tombácz, Dóra
Prorok, János
Tóth, András
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-07-14
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
We report the development and application of a pseudorabies virus-based system for delivery of troponeon, a fluorescent Ca2+ sensor to adult canine cardiomyocytes.
The efficacy of transduction was assessed by calculating the ratio of fluorescently labelled and nonlabelled cells in cell culture.
Interaction of the virus vector with electrophysiological properties of cardiomyocytes was evaluated by the analysis of transient outward current (Ito), kinetics of the intracellular Ca2+ transients, and cell shortening.
Functionality of transferred troponeon was verified by FRET analysis.
We demonstrated that the transfer efficiency of troponeon to cultured adult cardiac myocytes was virtually 100%.
We showed that even after four days neither the amplitude nor the kinetics of the Ito current was significantly changed and no major shifts occurred in parameters of [Ca2+]i transients.
Furthermore, we demonstrated that infection of cardiomyocytes with the virus did not affect the morphology, viability, and physiological attributes of cells.
American Psychological Association (APA)
Prorok, János& Kovács, Péter P.& Kristóf, Attila A.& Nagy, Norbert& Tombácz, Dóra& Tóth, Judit S.…[et al.]. 2009. Herpesvirus-Mediated Delivery of a Genetically Encoded Fluorescent Ca2+ Sensor to Canine Cardiomyocytes. BioMed Research International،Vol. 2009, no. 2009, pp.1-12.
https://search.emarefa.net/detail/BIM-1027092
Modern Language Association (MLA)
Kovács, Péter P.…[et al.]. Herpesvirus-Mediated Delivery of a Genetically Encoded Fluorescent Ca2+ Sensor to Canine Cardiomyocytes. BioMed Research International No. 2009 (2009), pp.1-12.
https://search.emarefa.net/detail/BIM-1027092
American Medical Association (AMA)
Prorok, János& Kovács, Péter P.& Kristóf, Attila A.& Nagy, Norbert& Tombácz, Dóra& Tóth, Judit S.…[et al.]. Herpesvirus-Mediated Delivery of a Genetically Encoded Fluorescent Ca2+ Sensor to Canine Cardiomyocytes. BioMed Research International. 2009. Vol. 2009, no. 2009, pp.1-12.
https://search.emarefa.net/detail/BIM-1027092
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1027092