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The Application of Restriction Landmark Genome Scanning Method for Surveillance of Non-Mendelian Inheritance in F1 Hybrids
Joint Authors
Takamiya, Tomoko
Paterson, Andrew H.
Okuizumi, Hisato
Murakami, Yasufumi
Iijima, Hiroshi
Noguchi, Tomotsugu
Hosobuchi, Saeko
Source
International Journal of Genomics
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-01-27
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
We analyzed inheritance of DNA methylation in reciprocal F1 hybrids (subsp.
japonica cv.
Nipponbare × subsp.
indica cv.
Kasalath) of rice (Oryza sativa L.) using restriction landmark genome scanning (RLGS), and detected differing RLGS spots between the parents and reciprocal F1 hybrids.
MspI/HpaII restriction sites in the DNA from these different spots were suspected to be heterozygously methylated in the Nipponbare parent.
These spots segregated in F1 plants, but did not segregate in selfed progeny of Nipponbare, showing non-Mendelian inheritance of the methylation status.
As a result of RT-PCR and sequencing, a specific allele of the gene nearest to the methylated sites was expressed in reciprocal F1 plants, showing evidence of biased allelic expression.
These results show the applicability of RLGS for scanning of non-Mendelian inheritance of DNA methylation and biased allelic expression.
American Psychological Association (APA)
Takamiya, Tomoko& Hosobuchi, Saeko& Noguchi, Tomotsugu& Paterson, Andrew H.& Iijima, Hiroshi& Murakami, Yasufumi…[et al.]. 2010. The Application of Restriction Landmark Genome Scanning Method for Surveillance of Non-Mendelian Inheritance in F1 Hybrids. International Journal of Genomics،Vol. 2009, no. 2009, pp.1-6.
https://search.emarefa.net/detail/BIM-1027116
Modern Language Association (MLA)
Takamiya, Tomoko…[et al.]. The Application of Restriction Landmark Genome Scanning Method for Surveillance of Non-Mendelian Inheritance in F1 Hybrids. International Journal of Genomics No. 2009 (2009), pp.1-6.
https://search.emarefa.net/detail/BIM-1027116
American Medical Association (AMA)
Takamiya, Tomoko& Hosobuchi, Saeko& Noguchi, Tomotsugu& Paterson, Andrew H.& Iijima, Hiroshi& Murakami, Yasufumi…[et al.]. The Application of Restriction Landmark Genome Scanning Method for Surveillance of Non-Mendelian Inheritance in F1 Hybrids. International Journal of Genomics. 2010. Vol. 2009, no. 2009, pp.1-6.
https://search.emarefa.net/detail/BIM-1027116
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1027116