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Neutrophils Compromise Retinal Pigment Epithelial Barrier Integrity
Joint Authors
Spee, Christine
Zhou, Peng
Ryan, Stephen J.
Kannan, Ram
Hinton, David R.
He, Shikun
Zhang, Ning
Zhou, Jiehao
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-12-28
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
We hypothesized that neutrophils and their secreted factors mediate breakdown of the integrity of the outer blood-retina-barrier by degrading the apical tight junctions of the retinal pigment epithelium (RPE).
The effect of activated neutrophils or neutrophil cell lysate on apparent permeability of bovine RPE-Choroid explants was evaluated by measuring [H3] mannitol flux in a modified Ussing chamber.
The expression of matrix metalloproteinase- (MMP-) 9 in murine peritoneal neutrophils, and the effects of neutrophils on RPE tight-junction protein expression were assessed by confocal microscopy and western blot.
Our results revealed that basolateral incubation of explants with neutrophils decreased occludin and ZO-1 expression at 1 and 3 hours and increased the permeability of bovine RPE-Choroid explants by >3-fold (P<.05).
Similarly, basolateral incubation of explants with neutrophil lysate decreased ZO-1 expression at 1 and 3 hours (P<.05) and increased permeability of explants by 75%.
Further, we found that neutrophils prominently express MMP-9 and that incubation of explants with neutrophils in the presence of anti-MMP-9 antibody inhibited the increase in permeability.
These data suggest that neutrophil-derived MMP-9 may play an important role in disrupting the integrity of the outer blood-retina barrier.
American Psychological Association (APA)
Zhou, Jiehao& He, Shikun& Zhang, Ning& Spee, Christine& Zhou, Peng& Ryan, Stephen J.…[et al.]. 2009. Neutrophils Compromise Retinal Pigment Epithelial Barrier Integrity. BioMed Research International،Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-1027139
Modern Language Association (MLA)
Zhou, Jiehao…[et al.]. Neutrophils Compromise Retinal Pigment Epithelial Barrier Integrity. BioMed Research International No. 2010 (Dec. 2010), pp.1-10.
https://search.emarefa.net/detail/BIM-1027139
American Medical Association (AMA)
Zhou, Jiehao& He, Shikun& Zhang, Ning& Spee, Christine& Zhou, Peng& Ryan, Stephen J.…[et al.]. Neutrophils Compromise Retinal Pigment Epithelial Barrier Integrity. BioMed Research International. 2009. Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-1027139
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1027139