Increased Expression of Ganglioside GM1 in Peripheral CD4+ T Cells Correlates Soluble Form of CD30 in Systemic Lupus Erythematosus Patients
Joint Authors
Sun, Wei
Umehara, Hisanori
Masaki, Yasufumi
Hu, Shaoxian
Dong, Lingli
Lei, Xiaomei
Yu, Yikai
Yang, Liu
Yamaguchi, Takuro
Tu, Wei
Chen, Fang
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-05-31
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Gangliosides GM1 is a good marker of membrane microdomains (lipid rafts) with important function in cellular activation processes.
In this study we found that GM1 expression on CD4+ T cells and memory T cells (CD45RO/CD4) were dramatic increased after stimulation with phytohaemagglutinin in vitro.
Next, we examined the GM1 expression on peripheral blood CD4+ T cells and CD8+ T cells from 44 patients with SLE and 28 healthy controls by flow cytometry.
GM1 expression was further analyzed with serum soluble CD30 (sCD30), IL-10, TNF-alpha and clinical parameters.
The mean fluorescence intensity of GM1 on CD4+ T cells from patients with SLE was significantly higher than those from healthy controls, but not on CD8+ T cells.
Increased expression of GM1 was more marked on CD4+/CD45RO+ memory T cells from active SLE patients.
Patients with SLE showed significantly elevated serum sCD30 and IL-10, but not TNF-alpha levels.
In addition, we found that enhanced GM1 expression on CD4+ T cells from patients with SLE positively correlated with high serum levels of sCD30 and IgG as well as disease activity (SLEDAI scores).
Our data suggested the potential role of aberrant lipid raft/GM1 on CD4+ T cells and sCD30 in the pathogenesis of SLE.
American Psychological Association (APA)
Dong, Lingli& Hu, Shaoxian& Chen, Fang& Lei, Xiaomei& Tu, Wei& Yu, Yikai…[et al.]. 2010. Increased Expression of Ganglioside GM1 in Peripheral CD4+ T Cells Correlates Soluble Form of CD30 in Systemic Lupus Erythematosus Patients. BioMed Research International،Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-1027147
Modern Language Association (MLA)
Dong, Lingli…[et al.]. Increased Expression of Ganglioside GM1 in Peripheral CD4+ T Cells Correlates Soluble Form of CD30 in Systemic Lupus Erythematosus Patients. BioMed Research International No. 2010 (Dec. 2010), pp.1-8.
https://search.emarefa.net/detail/BIM-1027147
American Medical Association (AMA)
Dong, Lingli& Hu, Shaoxian& Chen, Fang& Lei, Xiaomei& Tu, Wei& Yu, Yikai…[et al.]. Increased Expression of Ganglioside GM1 in Peripheral CD4+ T Cells Correlates Soluble Form of CD30 in Systemic Lupus Erythematosus Patients. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-1027147
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1027147