Regulation of Adrenal Aldosterone Production by Serine Protease Prostasin
Joint Authors
Kitamura, Kenichiro
Aritomi, Shizuka
Ko, Takehiro
Hayata, Manabu
Kakizoe, Yutaka
Wakida, Naoki
Uchimura, Kohei
Shiraishi, Naoki
Konda, Tomoyuki
Adachi, Masataka
Miyoshi, Taku
Tomita, Kimio
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-01-03
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
A serine protease prostasin has been demonstrated to have a pivotal role in the activation of the epithelial sodium channel.
Systemic administration of adenovirus carrying human prostasin gene in rats resulted in an increase in plasma prostasin and aldosterone levels.
However, the mechanism by which the elevation of prostasin levels in the systemic circulation stimulated the plasma aldosterone levels remains unknown.
Therefore, we examined if prostasin increases the aldosterone synthesis in a human adrenocortical cell line (H295R cells).
Luciferase assay using CYP11B2 promoter revealed that prostasin significantly increased the transcriptional activity of CYP11B2.
Prostasin significantly increased both CYP11B2 mRNA expression and aldosterone production in a dose-dependent manner.
Surprisingly, treatment with camostat mesilate, a potent prostasin inhibitor, had no effect on the aldosterone synthesis by prostasin and also a protease-dead mutant of prostasin significantly stimulated the aldosterone production.
A T-type/L-type calcium channel blocker and a protein kinase C (PKC) inhibitor significantly reduced the aldosterone synthesis by prostasin.
Our findings suggest a stimulatory effect of prostasin on the aldosterone synthesis by adrenal gland through the nonproteolytic action and indicate a new role of prostasin in the systemic circulation.
American Psychological Association (APA)
Ko, Takehiro& Kakizoe, Yutaka& Wakida, Naoki& Hayata, Manabu& Uchimura, Kohei& Shiraishi, Naoki…[et al.]. 2010. Regulation of Adrenal Aldosterone Production by Serine Protease Prostasin. BioMed Research International،Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-1027153
Modern Language Association (MLA)
Ko, Takehiro…[et al.]. Regulation of Adrenal Aldosterone Production by Serine Protease Prostasin. BioMed Research International No. 2010 (Dec. 2010), pp.1-9.
https://search.emarefa.net/detail/BIM-1027153
American Medical Association (AMA)
Ko, Takehiro& Kakizoe, Yutaka& Wakida, Naoki& Hayata, Manabu& Uchimura, Kohei& Shiraishi, Naoki…[et al.]. Regulation of Adrenal Aldosterone Production by Serine Protease Prostasin. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-1027153
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1027153