Herpesvirus BACs: Past, Present, and Future
Joint Authors
Warden, Charles
Tang, Qiyi
Zhu, Hua
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-08-22
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
The herpesviridae are a large family of DNA viruses with large and complicated genomes.
Genetic manipulation and the generation of recombinant viruses have been extremely difficult.
However, herpesvirus bacterial artificial chromosomes (BACs) that were developed approximately 10 years ago have become useful and powerful genetic tools for generating recombinant viruses to study the biology and pathogenesis of herpesviruses.
For example, BAC-directed deletion mutants are commonly used to determine the function and essentiality of viral genes.
In this paper, we discuss the creation of herpesvirus BACs, functional analyses of herpesvirus mutants, and future applications for studies of herpesviruses.
We describe commonly used methods to create and mutate herpesvirus BACs (such as site-directed mutagenesis and transposon mutagenesis).
We also evaluate the potential future uses of viral BACs, including vaccine development and gene therapy.
American Psychological Association (APA)
Warden, Charles& Tang, Qiyi& Zhu, Hua. 2010. Herpesvirus BACs: Past, Present, and Future. BioMed Research International،Vol. 2011, no. 2011, pp.1-16.
https://search.emarefa.net/detail/BIM-1027601
Modern Language Association (MLA)
Warden, Charles…[et al.]. Herpesvirus BACs: Past, Present, and Future. BioMed Research International No. 2011 (2011), pp.1-16.
https://search.emarefa.net/detail/BIM-1027601
American Medical Association (AMA)
Warden, Charles& Tang, Qiyi& Zhu, Hua. Herpesvirus BACs: Past, Present, and Future. BioMed Research International. 2010. Vol. 2011, no. 2011, pp.1-16.
https://search.emarefa.net/detail/BIM-1027601
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1027601