A Comparative BAC Map for the Gilthead Sea Bream (Sparus aurata L.)
Joint Authors
Kuhl, Heiner
Kotoulas, Georgios
Tine, Mbaye
Magoulas, Antonios
Sarropoulou, Elena
Reinhardt, Richard
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-09-28
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
This study presents the first comparative BAC map of the gilthead sea bream (Sparus aurata), a highly valuated marine aquaculture fish species in the Mediterranean.
High-throughput end sequencing of a BAC library yielded 92,468 reads (60.6 Mbp).
Comparative mapping was achieved by anchoring BAC end sequences to the three-spined stickleback (Gasterosteus aculeatus) genome.
BACs that were consistently ordered along the stickleback chromosomes accounted for 14,265 clones.
A fraction of 5,249 BACs constituted a minimal tiling path that covers 73.5% of the stickleback chromosomes and 70.2% of the genes that have been annotated.
The N50 size of 1,485 “BACtigs” consisting of redundant BACs is 337,253 bp.
The largest BACtig covers 2.15 Mbp in the stickleback genome.
According to the insert size distribution of mapped BACs the sea bream genome is 1.71-fold larger than the stickleback genome.
These results represent a valuable tool to researchers in the field and may support future projects to elucidate the whole sea bream genome.
American Psychological Association (APA)
Kuhl, Heiner& Sarropoulou, Elena& Tine, Mbaye& Kotoulas, Georgios& Magoulas, Antonios& Reinhardt, Richard. 2010. A Comparative BAC Map for the Gilthead Sea Bream (Sparus aurata L.). BioMed Research International،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-1027608
Modern Language Association (MLA)
Kuhl, Heiner…[et al.]. A Comparative BAC Map for the Gilthead Sea Bream (Sparus aurata L.). BioMed Research International No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-1027608
American Medical Association (AMA)
Kuhl, Heiner& Sarropoulou, Elena& Tine, Mbaye& Kotoulas, Georgios& Magoulas, Antonios& Reinhardt, Richard. A Comparative BAC Map for the Gilthead Sea Bream (Sparus aurata L.). BioMed Research International. 2010. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-1027608
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1027608