Protein Expression of Mesenchymal Stem Cells after Transfection of pcDNA3.1−-hVEGF165 by Ultrasound-Targeted Microbubble Destruction
Joint Authors
Zhang, Hong
Gao, Feng
Xie, Xiaojie
Jian'an, Wang
Xu, Qiyuan
You, Xiangdong
Pu, Zhaoxia
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-19
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
Ultrasound-targeted microbubble destruction (UTMD) has been proposed as a new technique for organ-specific gene transfer and drug delivery.
This study was performed to investigate the effect of UTMD on marrow mesenchymal stem cells (MSCs) transfected with pcDNA3.1−-hVEGF165.pcDNA3.1−-hVEGF165 were transfected into the third passage of MSCs, with or without UTMD under different ultrasound conditions.
Protein expression was quantified by hVEGF165-ELISA kit after transfection for 24, 48, and 72 hours.
UTMD-mediated transfection of MSCs yielded a significant protein expression.
UTMD of mechanic index (MI) 0.6 for 90 seconds led to the highest level of protein expression.
American Psychological Association (APA)
Pu, Zhaoxia& You, Xiangdong& Xu, Qiyuan& Gao, Feng& Xie, Xiaojie& Zhang, Hong…[et al.]. 2011. Protein Expression of Mesenchymal Stem Cells after Transfection of pcDNA3.1−-hVEGF165 by Ultrasound-Targeted Microbubble Destruction. BioMed Research International،Vol. 2011, no. 2011, pp.1-4.
https://search.emarefa.net/detail/BIM-1027629
Modern Language Association (MLA)
Pu, Zhaoxia…[et al.]. Protein Expression of Mesenchymal Stem Cells after Transfection of pcDNA3.1−-hVEGF165 by Ultrasound-Targeted Microbubble Destruction. BioMed Research International No. 2011 (2011), pp.1-4.
https://search.emarefa.net/detail/BIM-1027629
American Medical Association (AMA)
Pu, Zhaoxia& You, Xiangdong& Xu, Qiyuan& Gao, Feng& Xie, Xiaojie& Zhang, Hong…[et al.]. Protein Expression of Mesenchymal Stem Cells after Transfection of pcDNA3.1−-hVEGF165 by Ultrasound-Targeted Microbubble Destruction. BioMed Research International. 2011. Vol. 2011, no. 2011, pp.1-4.
https://search.emarefa.net/detail/BIM-1027629
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1027629
