Protection against Autoimmune Diabetes by Silkworm-Produced GFP-Tagged CTB-Insulin Fusion Protein
Joint Authors
Shi, Xiaowen
Zhang, Yaozhou
Wang, Wenfeng
Meng, Qiaohong
Jin, Yongfeng
Source
Journal of Immunology Research
Issue
Vol. 2011, Issue 2011 (31 Dec. 2010), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-06-30
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
In animals, oral administration of the cholera toxin B (CTB) subunit conjugated to the autoantigen insulin enhances the specific immune-unresponsive state.
This is called oral tolerance and is capable of suppressing autoimmune type 1 diabetes (T1D).
However, the process by which the CTB-insulin (CTB-INS) protein works as a therapy for T1D in vivo remains unclear.
Here, we successfully expressed a green fluorescent protein- (GFP-) tagged CTB-Ins (CTB-Ins-GFP) fusion protein in silkworms in a pentameric form that retained the native ability to activate the mechanism.
Oral administration of the CTB-Ins-GFP protein induced special tolerance, delayed the development of diabetic symptoms, and suppressed T1D onset in nonobese diabetic (NOD) mice.
Moreover, it increased the numbers of CD4+CD25+Foxp3+ T regulatory (Treg) cells in peripheral lymph tissues and affected the biological activity of spleen cells.
This study demonstrated that the CTB-Ins-GFP protein produced in silkworms acted as an oral protein vaccine, inducing immunological tolerance involving CD4+CD25+Foxp3+ Treg cells in treating T1D.
American Psychological Association (APA)
Meng, Qiaohong& Wang, Wenfeng& Shi, Xiaowen& Jin, Yongfeng& Zhang, Yaozhou. 2011. Protection against Autoimmune Diabetes by Silkworm-Produced GFP-Tagged CTB-Insulin Fusion Protein. Journal of Immunology Research،Vol. 2011, no. 2011, pp.1-14.
https://search.emarefa.net/detail/BIM-1027711
Modern Language Association (MLA)
Meng, Qiaohong…[et al.]. Protection against Autoimmune Diabetes by Silkworm-Produced GFP-Tagged CTB-Insulin Fusion Protein. Journal of Immunology Research Vol. 2011, no. 2011 (2010), pp.1-14.
https://search.emarefa.net/detail/BIM-1027711
American Medical Association (AMA)
Meng, Qiaohong& Wang, Wenfeng& Shi, Xiaowen& Jin, Yongfeng& Zhang, Yaozhou. Protection against Autoimmune Diabetes by Silkworm-Produced GFP-Tagged CTB-Insulin Fusion Protein. Journal of Immunology Research. 2011. Vol. 2011, no. 2011, pp.1-14.
https://search.emarefa.net/detail/BIM-1027711
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1027711