Improvement of Accuracy in Flow Immunosensor System by Introduction of Poly-2-[3-(methacryloylamino)propylammonio]ethyl 3-aminopropyl Phosphate
Joint Authors
Yasuzawa, Mikito
Futagami, Norimichi
Rikitake, Kotaro
Fuchiwaki, Yusuke
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-07-03
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
In order to improve the accuracy of immunosensor systems, poly-2-[3-(methacryloylamino)propylammonio]ethyl 3-aminopropyl phosphate (poly-3MAm3AP), which includes both phosphorylcholine and amino groups, was synthesized and applied to the preparation of antibody-immobilized beads.
Acting as an antibody-immobilizing material, poly-3MAm3AP is expected to significantly lower nonspecific adsorption due to the presence of the phosphorylcholine group and recognize large numbers of analytes due to the increase in antibody-immobilizing sites.
The elimination of nonspecific adsorption was compared between the formation of a blocking layer on antibody-immobilized beads and the introduction of a material to combine antibody with beads.
Determination with specific and nonspecific antibodies was then investigated for the estimation of signal-to-noise ratio.
Signal intensities with superior signal-to-noise ratios were obtained when poly-3MAm3AP was introduced.
This may be due to the increase in antibody-immobilizing sites and the extended space for antigen-antibody interaction resulting from the electrostatic repulsion of poly-3MAm3AP.
Thus, the application of poly-3MAm3AP coatings to immunoassay beads was able to improve the accuracy of flow immunosensor systems.
American Psychological Association (APA)
Fuchiwaki, Yusuke& Yasuzawa, Mikito& Futagami, Norimichi& Rikitake, Kotaro. 2011. Improvement of Accuracy in Flow Immunosensor System by Introduction of Poly-2-[3-(methacryloylamino)propylammonio]ethyl 3-aminopropyl Phosphate. Journal of Sensors،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-1027716
Modern Language Association (MLA)
Fuchiwaki, Yusuke…[et al.]. Improvement of Accuracy in Flow Immunosensor System by Introduction of Poly-2-[3-(methacryloylamino)propylammonio]ethyl 3-aminopropyl Phosphate. Journal of Sensors No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-1027716
American Medical Association (AMA)
Fuchiwaki, Yusuke& Yasuzawa, Mikito& Futagami, Norimichi& Rikitake, Kotaro. Improvement of Accuracy in Flow Immunosensor System by Introduction of Poly-2-[3-(methacryloylamino)propylammonio]ethyl 3-aminopropyl Phosphate. Journal of Sensors. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-1027716
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1027716