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Stem Cell Heterogeneity of Mononucleated Cells from Murine Peripheral Blood: Molecular Analysis
Joint Authors
Yazid, Muhammad Dain
Zainal Ariffin, Zaidah
Megat Abdul Wahab, Rohaya
Senafi, Sahidan
Zainal Ariffin, Shahrul Hisham
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-11-03
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
The main purpose of this paper was to determine the heterogeneity of primary isolated mononucleated cells that originated from the peripheral blood system by observing molecular markers.
The isolated cells were cultured in complete medium for 4 to 7 days prior to the separation of different cell types, that is, adherent and suspension.
Following a total culture time of 14 days, adherent cells activated the Cd105 gene while suspension cells activated the Sca-1 gene.
Both progenitor markers, Cbfa-1 and Ostf-1, were inactivated in both suspension and adherent cells after 14-day culture compared to cells cultured 3 days in designated differentiation medium.
In conclusion, molecular analyses showed that primary mononucleated cells are heterogeneous, consisting of hematopoietic stem cells (suspension) and mesenchymal stem cells (adherent) while both cells contained no progenitor cells.
American Psychological Association (APA)
Yazid, Muhammad Dain& Zainal Ariffin, Shahrul Hisham& Senafi, Sahidan& Zainal Ariffin, Zaidah& Megat Abdul Wahab, Rohaya. 2011. Stem Cell Heterogeneity of Mononucleated Cells from Murine Peripheral Blood: Molecular Analysis. The Scientific World Journal،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-1027868
Modern Language Association (MLA)
Yazid, Muhammad Dain…[et al.]. Stem Cell Heterogeneity of Mononucleated Cells from Murine Peripheral Blood: Molecular Analysis. The Scientific World Journal No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-1027868
American Medical Association (AMA)
Yazid, Muhammad Dain& Zainal Ariffin, Shahrul Hisham& Senafi, Sahidan& Zainal Ariffin, Zaidah& Megat Abdul Wahab, Rohaya. Stem Cell Heterogeneity of Mononucleated Cells from Murine Peripheral Blood: Molecular Analysis. The Scientific World Journal. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-1027868
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1027868