Comparing the Heterogeneity of Copper-Binding Characteristics for Two Different-Sized Soil Humic Acid Fractions Using Fluorescence Quenching Combined with 2D-COS
Joint Authors
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-24
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
Heterogeneous distributions of copper-binding characteristics were compared for two ultrafiltered size fractions of a soil HA using fluorescence quenching combined with two-dimensional correlation spectroscopy (2D-COS).
The apparent shapes of the original synchronous fluorescence spectra and the extent of the fluorescence quenching upon the addition of copper were similar for the two fractions.
The stability constants calculated at their highest peaks were not significantly different.
However, the 2D-COS results revealed that the fluorescence quenching behaviors were strongly affected by the associated wavelengths and the fraction's size.
The spectral change preferentially occurred in the wavelength order of 467 nm → 451 nm → 357 nm for the 1–10 K fraction and of 376 nm → 464 nm for the >100 K fraction.
The extent of the binding affinities exactly followed the sequential orders interpreted from the 2D-COS, and they exhibited the distinctive ranges of the logarithmic values from 5.86 to 4.91 and from 6.48 to 5.95 for the 1–10 K and the >100 K fractions, respectively.
Our studies demonstrated that fluorescence quenching combined with 2D-COS could be successfully utilized to give insight into the chemical heterogeneity associated with metal-binding sites within the relatively homogeneous HA size fractions.
American Psychological Association (APA)
Hur, Jin& Lee, Bo-Mi. 2011. Comparing the Heterogeneity of Copper-Binding Characteristics for Two Different-Sized Soil Humic Acid Fractions Using Fluorescence Quenching Combined with 2D-COS. The Scientific World Journal،Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-1027887
Modern Language Association (MLA)
Hur, Jin& Lee, Bo-Mi. Comparing the Heterogeneity of Copper-Binding Characteristics for Two Different-Sized Soil Humic Acid Fractions Using Fluorescence Quenching Combined with 2D-COS. The Scientific World Journal No. 2011 (2011), pp.1-12.
https://search.emarefa.net/detail/BIM-1027887
American Medical Association (AMA)
Hur, Jin& Lee, Bo-Mi. Comparing the Heterogeneity of Copper-Binding Characteristics for Two Different-Sized Soil Humic Acid Fractions Using Fluorescence Quenching Combined with 2D-COS. The Scientific World Journal. 2011. Vol. 2011, no. 2011, pp.1-12.
https://search.emarefa.net/detail/BIM-1027887
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1027887