Small Interference RNA Targeting TLR4 Gene Effectively Attenuates Pulmonary Inflammation in a Rat Model

Joint Authors

Yu, Weifeng
Liu, Yantao
Miao, Xuerong
Sun, Yuming
Wu, Feixiang
Lv, Xin

Source

BioMed Research International

Issue

Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2012-02-06

Country of Publication

Egypt

No. of Pages

8

Main Subjects

Medicine

Abstract EN

Objective.

The present study was to investigate the feasibility of adenovirus-mediated small interference RNA (siRNA) targeting Toll-like receptor 4 (TLR4) gene in ameliorating lipopolysaccharide- (LPS-) induced acute lung injury (ALI).

Methods.

In vitro, alveolar macrophages (AMs) were treated with Ad-siTLR4 and Ad-EFGP, respectively, for 12 h, 24 h, and 48 h, and then with LPS (100 ng/mL) for 2 h, and the function and expression of TLR4 were evaluated.

In vivo, rats received intratracheal injection of 300 μL of normal saline (control group), 300 μL of Ad-EGFP (Ad-EGFP group), or 300 μL of Ad-siTLR4 (Ad-siTLR4 group) and then were intravenously treated with LPS (50 mg/kg) to induce ALI.

Results.

Ad-siTLR4 treatment significantly reduced TLR4 expression and production of proinflammatory cytokines following LPS treatment both in vitro and in vivo.

Significant alleviation of tissue edema, microvascular protein leakage, and neutrophil infiltration was observed in the AdsiTLR4-treated animals.

Conclusion.

TLR4 plays a critical role in LPS-induced ALI, and transfection of Ad-siTLR4 can effectively downregulate TLR4 expression in vitro and in vivo, accompanied by alleviation of LPS-induced lung injury.

These findings suggest that TLR4 may serve as a potential target in the treatment of ALI and RNA interfering targeting TLR4 expression represents a therapeutic strategy.

American Psychological Association (APA)

Wu, Feixiang& Liu, Yantao& Lv, Xin& Miao, Xuerong& Sun, Yuming& Yu, Weifeng. 2012. Small Interference RNA Targeting TLR4 Gene Effectively Attenuates Pulmonary Inflammation in a Rat Model. BioMed Research International،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-1027917

Modern Language Association (MLA)

Wu, Feixiang…[et al.]. Small Interference RNA Targeting TLR4 Gene Effectively Attenuates Pulmonary Inflammation in a Rat Model. BioMed Research International No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-1027917

American Medical Association (AMA)

Wu, Feixiang& Liu, Yantao& Lv, Xin& Miao, Xuerong& Sun, Yuming& Yu, Weifeng. Small Interference RNA Targeting TLR4 Gene Effectively Attenuates Pulmonary Inflammation in a Rat Model. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-1027917

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1027917