Betulinic Acid Inhibits Growth of Cultured Vascular Smooth Muscle Cells In Vitro by Inducing G1 Arrest and Apoptosis
Joint Authors
Alitheen, Noorjahan Banu Mohamed
Vadivelu, Raja Kumar
Hamid, Muhajir
Ali, Abdul Manaf
Yeap, Swee Keong
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-27
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Betulinic acid is a widely available plant-derived triterpene which is reported to possess selective cytotoxic activity against cancer cells of neuroectodermal origin and leukemia.
However, the potential of betulinic acid as an antiproliferative and cytotoxic agent on vascular smooth muscle (VSMC) is still unclear.
This study was carried out to demonstrate the antiproliferative and cytotoxic effect of betulinic acid on VSMCs using 3-[4,5-dimethylthizol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry cell cycle assay, BrdU proliferation assay, acridine orange/propidium iodide staining, and comet assay.
Result from MTT and BrdU assays indicated that betulinic acid was able to inhibit the growth and proliferation of VSMCs in a dose-dependent manner with IC50 of 3.8 μg/mL significantly (P<0.05).
Nevertheless, betulinic acid exhibited G1 cell cycle arrest in flow cytometry cell cycle profiling and low level of DNA damage against VSMC in acridine orange/propidium iodide and comet assay after 24 h of treatment.
In conclusion, betulinic acid induced G1 cell cycle arrest and dose-dependent DNA damage on VSMC.
American Psychological Association (APA)
Vadivelu, Raja Kumar& Yeap, Swee Keong& Ali, Abdul Manaf& Hamid, Muhajir& Alitheen, Noorjahan Banu Mohamed. 2012. Betulinic Acid Inhibits Growth of Cultured Vascular Smooth Muscle Cells In Vitro by Inducing G1 Arrest and Apoptosis. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-1028187
Modern Language Association (MLA)
Vadivelu, Raja Kumar…[et al.]. Betulinic Acid Inhibits Growth of Cultured Vascular Smooth Muscle Cells In Vitro by Inducing G1 Arrest and Apoptosis. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-1028187
American Medical Association (AMA)
Vadivelu, Raja Kumar& Yeap, Swee Keong& Ali, Abdul Manaf& Hamid, Muhajir& Alitheen, Noorjahan Banu Mohamed. Betulinic Acid Inhibits Growth of Cultured Vascular Smooth Muscle Cells In Vitro by Inducing G1 Arrest and Apoptosis. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-1028187
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1028187