The Cytotoxicity Mechanism of 6-Shogaol-Treated HeLa Human Cervical Cancer Cells Revealed by Label-Free Shotgun Proteomics and Bioinformatics Analysis

Abstract EN

Cervical cancer is one of the most common cancers among women in the world.

6-Shogaol is a natural compound isolated from the rhizome of ginger (Zingiber officinale).

In this paper, we demonstrated that 6-shogaol induced apoptosis and G2/M phase arrest in human cervical cancer HeLa cells.

Endoplasmic reticulum stress and mitochondrial pathway were involved in 6-shogaol-mediated apoptosis.

Proteomic analysis based on label-free strategy by liquid chromatography chip quadrupole time-of-flight mass spectrometry was subsequently proposed to identify, in a non-target-biased manner, the molecular changes in cellular proteins in response to 6-shogaol treatment.

A total of 287 proteins were differentially expressed in response to 24 h treatment with 15 μM 6-shogaol in HeLa cells.

Significantly changed proteins were subjected to functional pathway analysis by multiple analyzing software.

Ingenuity pathway analysis (IPA) suggested that 14-3-3 signaling is a predominant canonical pathway involved in networks which may be significantly associated with the process of apoptosis and G2/M cell cycle arrest induced by 6-shogaol.

In conclusion, this work developed an unbiased protein analysis strategy by shotgun proteomics and bioinformatics analysis.

Data observed provide a comprehensive analysis of the 6-shogaol-treated HeLa cell proteome and reveal protein alterations that are associated with its anticancer mechanism.