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Mitochondria Superoxide Anion Production Contributes to Geranylgeraniol-Induced Death in Leishmania amazonensis
Joint Authors
Desoti, Vânia Cristina
Ueda-Nakamura, Tânia
Lopes, Milene Valéria
Caleare, Angelo de Oliveira
Silva, Sueli de Oliveira
Nakamura, Celso Vataru
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-04
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Here we demonstrate the activity of geranylgeraniol, the major bioactive constituent from seeds of Bixa orellana, against Leishmania amazonensis.
Geranylgeraniol was identified through 1H and 13C nuclear magnetic resonance imaging and DEPT.
The compound inhibited the promastigote and intracellular amastigote forms, with IC50 of 11±1.0 and 17.5±0.7 μg/mL, respectively.
This compound was also more toxic to parasites than to macrophages and did not cause lysis in human blood cells.
Morphological and ultrastructural changes induced by geranylgeraniol were observed in the protozoan by electronic microscopy and included mainly mitochondria alterations and an abnormal chromatin condensation in the nucleus.
These alterations were confirmed by Rh 123 and TUNEL assays.
Additionally, geranylgeraniol induces an increase in superoxide anion production.
Collectively, our in vitro studies indicate geranylgeraniol as a selective antileishmanial that appears to be mediated by apoptosis-like cell death.
American Psychological Association (APA)
Lopes, Milene Valéria& Desoti, Vânia Cristina& Caleare, Angelo de Oliveira& Ueda-Nakamura, Tânia& Silva, Sueli de Oliveira& Nakamura, Celso Vataru. 2012. Mitochondria Superoxide Anion Production Contributes to Geranylgeraniol-Induced Death in Leishmania amazonensis. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-1028240
Modern Language Association (MLA)
Lopes, Milene Valéria…[et al.]. Mitochondria Superoxide Anion Production Contributes to Geranylgeraniol-Induced Death in Leishmania amazonensis. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-1028240
American Medical Association (AMA)
Lopes, Milene Valéria& Desoti, Vânia Cristina& Caleare, Angelo de Oliveira& Ueda-Nakamura, Tânia& Silva, Sueli de Oliveira& Nakamura, Celso Vataru. Mitochondria Superoxide Anion Production Contributes to Geranylgeraniol-Induced Death in Leishmania amazonensis. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-1028240
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1028240