Antiangiogenic Effects and Therapeutic Targets of Azadirachta indica Leaf Extract in Endothelial Cells
Joint Authors
Donkena, Krishna Vanaja
Kohli, Manish
Mahapatra, Saswati
Klee, Eric W.
Holmes, Michael W.
Tindall, Donald J.
Young, Charles Y. F.
Karnes, R. Jeffrey
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-22
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Azadirachta indica (common name: neem) leaves have been found to possess immunomodulatory, anti-inflammatory and anti-carcinogenic properties.
The present study evaluates anti-angiogenic potential of ethanol extract of neem leaves (EENL) in human umbilical vein endothelial cells (HUVECs).
Treatment of HUVECs with EENL inhibited VEGF induced angiogenic response in vitro and in vivo.
The in vitro proliferation, invasion and migration of HUVECs were suppressed with EENL.
Nuclear fragmentation and abnormally small mitochondria with dilated cristae were observed in EENL treated HUVECs by transmission electron microscopy.
Genome-wide mRNA expression profiling after treatment with EENL revealed differentially regulated genes.
Expression changes of the genes were validated by quantitative real-time polymerase chain reaction.
Additionally, increase in the expression of HMOX1, ATF3 and EGR1 proteins were determined by immunoblotting.
Analysis of the compounds in the EENL by mass spectrometry suggests the presence of nimbolide, 2′,3′-dehydrosalannol, 6-desacetyl nimbinene and nimolinone.
We further confirmed antiproliferative activity of nimbolide and 2′,3′-dehydrosalannol in HUVECs.
Our results suggest that EENL by regulating the genes involved in cellular development and cell death functions could control cell proliferation, attenuate the stimulatory effects of VEGF and exert antiangiogenic effects.
EENL treatment could have a potential therapeutic role during cancer progression.
American Psychological Association (APA)
Mahapatra, Saswati& Young, Charles Y. F.& Kohli, Manish& Karnes, R. Jeffrey& Klee, Eric W.& Holmes, Michael W.…[et al.]. 2012. Antiangiogenic Effects and Therapeutic Targets of Azadirachta indica Leaf Extract in Endothelial Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-14.
https://search.emarefa.net/detail/BIM-1028246
Modern Language Association (MLA)
Mahapatra, Saswati…[et al.]. Antiangiogenic Effects and Therapeutic Targets of Azadirachta indica Leaf Extract in Endothelial Cells. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-14.
https://search.emarefa.net/detail/BIM-1028246
American Medical Association (AMA)
Mahapatra, Saswati& Young, Charles Y. F.& Kohli, Manish& Karnes, R. Jeffrey& Klee, Eric W.& Holmes, Michael W.…[et al.]. Antiangiogenic Effects and Therapeutic Targets of Azadirachta indica Leaf Extract in Endothelial Cells. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-14.
https://search.emarefa.net/detail/BIM-1028246
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1028246