![](/images/graphics-bg.png)
Hwangryun-Haedok-Tang Fermented with Lactobacillus casei Suppresses Ovariectomy-Induced Bone Loss
Joint Authors
Ma, Jin Yeul
Shim, Ki-Shuk
Cho, Chang-Won
Kim, Han Sung
Seo, Dong-Hyun
Ha, Hyunil
Kim, Taesoo
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-02
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Hwangryun-haedok-tang (HRT) is the common recipe in traditional Asian medicine, and microbial fermentation is used for the conventional methods for processing traditional medicine.
We investigated the inhibitory effect of the n-butanol fraction of HRT (HRT-BU) and fHRT (fHRT-BU) on the RANKL-induced osteoclastogenesis in bone-marrow-derived macrophages.
mRNA expression of osteoclastogenesis-related genes were evaluated by real-time QPCR.
The activation of signaling pathways was determined by western blot analysis.
The marker compounds of HRT-BU and fHRT-BU were analyzed by HPLC.
The inhibitory effect of HRT or fHRT on ovariectomy-induced bone loss were evaluated using OVX rats with orally administered HRT, fHRT (300, 1000 mg/kg), or its vehicle for 12 weeks.
fHRT-BU significantly inhibited RANKL-induced osteoclastogenesis, and phosphorylation of p38, IKKα/β, and NF-κBp65 compared to HRT-BU.
In addition, fHRT-BU also significantly inhibited the mRNA expression of Nfκb2, TNF-α, NFATc1, TRAP, ATPv0d2, and cathepsin K.
Furthermore, administration of fHRT had a greater effect on the increase of BMD, and greater improved bone microstructure of the femora than that of HRT in ovariectomy rats.
This study demonstrated that bacterial fermentation enhances the inhibitory effect of HRT on osteoclastogenesis and bone loss.
These results suggest that fermented HRT might have the beneficial effects on bone disease by inhibiting osteoclastogenesis.
American Psychological Association (APA)
Shim, Ki-Shuk& Kim, Taesoo& Ha, Hyunil& Cho, Chang-Won& Kim, Han Sung& Seo, Dong-Hyun…[et al.]. 2012. Hwangryun-Haedok-Tang Fermented with Lactobacillus casei Suppresses Ovariectomy-Induced Bone Loss. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-1028257
Modern Language Association (MLA)
Shim, Ki-Shuk…[et al.]. Hwangryun-Haedok-Tang Fermented with Lactobacillus casei Suppresses Ovariectomy-Induced Bone Loss. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-1028257
American Medical Association (AMA)
Shim, Ki-Shuk& Kim, Taesoo& Ha, Hyunil& Cho, Chang-Won& Kim, Han Sung& Seo, Dong-Hyun…[et al.]. Hwangryun-Haedok-Tang Fermented with Lactobacillus casei Suppresses Ovariectomy-Induced Bone Loss. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-1028257
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1028257