Ethanol Extracts of Fruiting Bodies of Antrodia cinnamomea Suppress CL1-5 Human Lung Adenocarcinoma Cells Migration by Inhibiting Matrix Metalloproteinase-29 through ERK, JNK, p38, and PI3KAkt Signaling Pathways
Joint Authors
Sheu, Ming-Jyh
Liu, Fon-Chang
Chou, Pei-Yu
Chang, Wun-Shaing Wayne
Wu, Chieh-Hsi
Chen, Ying-Yi
Huang, Guan-Jhong
Chien, Yi-Chung
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-21
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Cancer metastasis is a primary cause of cancer death.
Antrodia cinnamomea (A.
cinnamomea), a medicinal mushroom in Taiwan, has shown antioxidant and anticancer activities.
In this study, we first observed that ethanol extract of fruiting bodies of A.
cinnamomea (EEAC) exerted a concentration-dependent inhibitory effect on migration and motility of the highly metastatic CL1-5 cells in the absence of cytotoxicity.
The results of a gelatin zymography assay showed that A.
cinnamomea suppressed the activities of matrix metalloproteinase-(MMP-) 2 and MMP-9 in a concentration-dependent manner.
Western blot results demonstrated that treatment with A.
cinnamomea decreased the expression of MMP-9 and MMP-2; while the expression of the endogenous inhibitors of these proteins, that is, tissue inhibitors of MMP (TIMP-1 and TIMP-2) increased.
Further investigation revealed that A.
cinnamomea suppressed the phosphorylation of ERK1/2, p38, and JNK1/2.
A.
cinnamomea also suppressed the expressions of PI3K and phosphorylation of Akt.
Furthermore, treatment of CL1-5 cells with inhibitors specific for PI3K (LY 294002), ERK1/2 (PD98059), JNK (SP600125), and p38 MAPK (SB203580) decreased the expression of MMP-2 and MMP-9.
This is the first paper confirming the antimigration activity of this potentially beneficial mushroom against human lung adenocarcinoma CL1-5 cancer cells.
American Psychological Association (APA)
Chen, Ying-Yi& Liu, Fon-Chang& Chou, Pei-Yu& Chien, Yi-Chung& Chang, Wun-Shaing Wayne& Huang, Guan-Jhong…[et al.]. 2012. Ethanol Extracts of Fruiting Bodies of Antrodia cinnamomea Suppress CL1-5 Human Lung Adenocarcinoma Cells Migration by Inhibiting Matrix Metalloproteinase-29 through ERK, JNK, p38, and PI3KAkt Signaling Pathways. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-1028280
Modern Language Association (MLA)
Chen, Ying-Yi…[et al.]. Ethanol Extracts of Fruiting Bodies of Antrodia cinnamomea Suppress CL1-5 Human Lung Adenocarcinoma Cells Migration by Inhibiting Matrix Metalloproteinase-29 through ERK, JNK, p38, and PI3KAkt Signaling Pathways. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-1028280
American Medical Association (AMA)
Chen, Ying-Yi& Liu, Fon-Chang& Chou, Pei-Yu& Chien, Yi-Chung& Chang, Wun-Shaing Wayne& Huang, Guan-Jhong…[et al.]. Ethanol Extracts of Fruiting Bodies of Antrodia cinnamomea Suppress CL1-5 Human Lung Adenocarcinoma Cells Migration by Inhibiting Matrix Metalloproteinase-29 through ERK, JNK, p38, and PI3KAkt Signaling Pathways. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-1028280
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1028280