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Apoptosis Induction in Primary Human Colorectal Cancer Cell Lines and Retarded Tumor Growth in SCID Mice by Sulforaphane
Joint Authors
Wu, June-Fu
Hsu, Yi-Chiang
Chen, Ming-Jenn
Tang, Wei-Yu
Hsu, Che-Wei
Tsai, Ya-Ting
Lin, Chen-Wei
Cheng, Ya-Min
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-06-06
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
We have investigated the anticancer effects of the dietary isothiocyanate sulforaphane (SFN) on colorectal cancer (CRC), using primary cancer cells lines isolated from five Taiwanese colorectal cancer patients as the model for colorectal cancer.
SFN-treated cells accumulated in metaphase (SFN 6.25 μM) and subG1 (SFN 12.5 and 25 μM) as determined by flow cytometry.
In addition, treated cells showed nuclear apoptotic morphology that coincided with an activation of caspase-3, and loss of mitochondrial membrane potential (ΔΨm).
Incubations at higher SFN doses (12.5 and 25 μM) resulted in cleavage of procaspase-3 and elevated caspase-2, -3, -8, and -9 activity, suggesting that the induction of apoptosis and the sulforaphane-induced mitosis delay at the lower dose are independently regulated.
Daily SFN s.c.
injections (400 micromol/kg/d for 3 weeks) in severe combined immunodeficient mice with primary human CRC (CP1 to CP5) s.c.
tumors resulted in a decrease of mean tumor weight by 70% compared with vehicle-treated controls.
Our findings suggest that, in addition to the known effects on cancer prevention, sulforaphane may have antitumor activity in established colorectal cancer.
American Psychological Association (APA)
Chen, Ming-Jenn& Tang, Wei-Yu& Hsu, Che-Wei& Tsai, Ya-Ting& Wu, June-Fu& Lin, Chen-Wei…[et al.]. 2011. Apoptosis Induction in Primary Human Colorectal Cancer Cell Lines and Retarded Tumor Growth in SCID Mice by Sulforaphane. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-1028300
Modern Language Association (MLA)
Chen, Ming-Jenn…[et al.]. Apoptosis Induction in Primary Human Colorectal Cancer Cell Lines and Retarded Tumor Growth in SCID Mice by Sulforaphane. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-13.
https://search.emarefa.net/detail/BIM-1028300
American Medical Association (AMA)
Chen, Ming-Jenn& Tang, Wei-Yu& Hsu, Che-Wei& Tsai, Ya-Ting& Wu, June-Fu& Lin, Chen-Wei…[et al.]. Apoptosis Induction in Primary Human Colorectal Cancer Cell Lines and Retarded Tumor Growth in SCID Mice by Sulforaphane. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-1028300
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1028300