Red Ginseng Extract Attenuates Kainate-Induced Excitotoxicity by Antioxidative Effects

Abstract EN

This study investigated the neuroprotective activity of red ginseng extract (RGE, Panax ginseng, C.

A.

Meyer) against kainic acid- (KA-) induced excitotoxicity in vitro and in vivo.

In hippocampal cells, RGE inhibited KA-induced excitotoxicity in a dose-dependent manner as measured by the MTT assay.

To study the possible mechanisms of the RGE-mediated neuroprotective effect against KA-induced cytotoxicity, we examined the levels of intracellular reactive oxygen species (ROS) and [Ca2+]i in cultured hippocampal neurons and found that RGE treatment dose-dependently inhibited intracellular ROS and [Ca2+]i elevation.

Oral administration of RGE (30 and 200 mg/kg) in mice decreased the malondialdehyde (MDA) level induced by KA injection (30 mg/kg, i.p.).

In addition, similar results were obtained after pretreatment with the radical scavengers Trolox and N, N′-dimethylthiourea (DMTU).

Finally, after confirming the protective effect of RGE on hippocampal brain-derived neurotropic factor (BDNF) protein levels, we found that RGE is active compounds mixture in KA-induced hippocampal mossy-fiber function improvement.

Furthermore, RGE eliminated 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, and the IC50 was approximately 10 mg/ml.

The reductive activity of RGE, as measured by reaction with hydroxyl radical (•OH), was similar to trolox.

The second-order rate constant of RGE for •OH was 3.5–4.5×109 M−1·S−1.

Therefore, these results indicate that RGE possesses radical reduction activity and alleviates KA-induced excitotoxicity by quenching ROS in hippocampal neurons.