Gallic Acid Enriched Fraction of Phyllanthus emblica Potentiates Indomethacin-Induced Gastric Ulcer Healing via e-NOS-Dependent Pathway
Joint Authors
Bandyopadhyay, Sandip K.
Chatterjee, Sirshendu
Biswas, Angshuman
Bhattacharya, Sayanti
Chatterjee, Ananya
Chattopadhyay, Subrata
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-08-26
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
The healing activity of gallic acid enriched ethanolic extract (GAE) of Phyllanthus emblica fruits (amla) against the indomethacin-induced gastric ulceration in mice was investigated.
The activity was correlated with the ability of GAE to alter the cyclooxygenase- (COX-) dependent healing pathways.
Histology of the stomach tissues revealed maximum ulceration on the 3rd day after indomethacin (18 mg/kg, single dose) administration that was associated with significant increase in inflammatory factors, namely, mucosal myeloperoxidase (MPO) activity and inducible nitric oxide synthase (i-NOS) expression.
Proangiogenic parameters such as the levels of prostaglandin (PG) E2, vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), von Willebrand Factor VIII, and endothelial NOS (e-NOS) were downregulated by indomethacin.
Treatment with GAE (5 mg/kg/day) and omeprazole (3 mg/kg/day) for 3 days led to effective healing of the acute ulceration, while GAE could reverse the indomethacin-induced proinflammatory changes of the designated biochemical parameters.
The ulcer healing activity of GAE was, however, compromised by coadministration of the nonspecific NOS inhibitor, N-nitro-L-arginine methyl ester (L-NAME), but not the i-NOS-specific inhibitor, L-N6-(1-iminoethyl) lysine hydrochloride (L-NIL).
Taken together, these results suggested that the GAE treatment accelerates ulcer healing by inducing PGE2 synthesis and augmenting e-NOS/i-NOS ratio.
American Psychological Association (APA)
Chatterjee, Ananya& Chatterjee, Sirshendu& Biswas, Angshuman& Bhattacharya, Sayanti& Chattopadhyay, Subrata& Bandyopadhyay, Sandip K.. 2012. Gallic Acid Enriched Fraction of Phyllanthus emblica Potentiates Indomethacin-Induced Gastric Ulcer Healing via e-NOS-Dependent Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-1028345
Modern Language Association (MLA)
Chatterjee, Ananya…[et al.]. Gallic Acid Enriched Fraction of Phyllanthus emblica Potentiates Indomethacin-Induced Gastric Ulcer Healing via e-NOS-Dependent Pathway. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-13.
https://search.emarefa.net/detail/BIM-1028345
American Medical Association (AMA)
Chatterjee, Ananya& Chatterjee, Sirshendu& Biswas, Angshuman& Bhattacharya, Sayanti& Chattopadhyay, Subrata& Bandyopadhyay, Sandip K.. Gallic Acid Enriched Fraction of Phyllanthus emblica Potentiates Indomethacin-Induced Gastric Ulcer Healing via e-NOS-Dependent Pathway. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-1028345
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1028345