Curcumin-Induced Apoptosis in Human Hepatocellular Carcinoma J5 Cells: Critical Role of Ca+2-Dependent Pathway
Joint Authors
Lin, Wuu-Jyh
Chung, Jing-Gung
Wang, Wei-Hsun
Chiang, I-Tsang
Ding, Kuke
Lin, Song-Shei
Hwang, Jeng-Jong
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-01
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The antitumor effects of curcumin, a natural biologically active compound extracted from rhizomes of Curcuma longa, have been studied in many cancer cell types including human hepatocellular carcinoma (HCC).
Here, we investigated the effects of Ca2+ on curcumin-induced apoptosis in human HCC J5 cells.
The abrogation of mitochondrial membrane potential (ΔΨm), the increase of reactive oxygen species (ROS) production, and calcium release were demonstrated with flow cytometry as early as 15 minutes after curcumin treatment.
In addition, an increase level of cytochrome c in the cytoplasm which led to DNA fragmentation was observed.
To verify the role of Ca2+ in curcumin-induced apoptosis, 1,2-bis(o-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid (BAPTA), an intracellular calcium chelator, was applied.
Cell viability was increased, but ΔΨm, ROS production, activation of caspase 3, and cell death were decreased in J5 cells pretreated with BAPTA for 2 h followed by the treatment of 25 μM curcumin.
These results suggest that the curcumin-induced apoptosis in human HCC J5 cells is via mitochondria-dependent pathway and is closely related to the level of intracellular accumulation of calcium.
American Psychological Association (APA)
Wang, Wei-Hsun& Chiang, I-Tsang& Ding, Kuke& Chung, Jing-Gung& Lin, Wuu-Jyh& Lin, Song-Shei…[et al.]. 2012. Curcumin-Induced Apoptosis in Human Hepatocellular Carcinoma J5 Cells: Critical Role of Ca+2-Dependent Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-1028370
Modern Language Association (MLA)
Wang, Wei-Hsun…[et al.]. Curcumin-Induced Apoptosis in Human Hepatocellular Carcinoma J5 Cells: Critical Role of Ca+2-Dependent Pathway. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-1028370
American Medical Association (AMA)
Wang, Wei-Hsun& Chiang, I-Tsang& Ding, Kuke& Chung, Jing-Gung& Lin, Wuu-Jyh& Lin, Song-Shei…[et al.]. Curcumin-Induced Apoptosis in Human Hepatocellular Carcinoma J5 Cells: Critical Role of Ca+2-Dependent Pathway. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-1028370
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1028370