Retinoic Acid Induces Apoptosis of Prostate Cancer DU145 Cells through Cdk5 Overactivation
Joint Authors
Chen, Chuan-Mu
Chen, Mei-Chih
Lin, Eugene
Ku, Chien-Te
Huang, Chih-Yang
Hsu, Shih-Lan
Lin, Ho
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-13
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Retinoic acid (RA) has been believed to be an anticancer drug for a long history.
However, the molecular mechanisms of RA actions on cancer cells remain diverse.
In this study, the dose-dependent inhibition of RA on DU145 cell proliferation was identified.
Interestingly, RA treatment triggered p35 cleavage (p25 formation) and Cdk5 overactivation, and all could be blocked by Calpain inhibitor, Calpeptin (CP).
Subsequently, RA-triggered DU145 apoptosis detected by sub-G1 phase accumulation and Annexin V staining could also be blocked by CP treatment.
Furthermore, RA-triggered caspase 3 activation and following Cdk5 over-activation were destroyed by treatments of both CP and Cdk5 knockdown.
In conclusion, we report a new mechanism in which RA could cause apoptosis of androgen-independent prostate cancer cells through p35 cleavage and Cdk5 over-activation.
This finding may contribute to constructing a clearer image of RA function and bring RA as a valuable chemoprevention agent for prostate cancer patients.
American Psychological Association (APA)
Chen, Mei-Chih& Huang, Chih-Yang& Hsu, Shih-Lan& Lin, Eugene& Ku, Chien-Te& Lin, Ho…[et al.]. 2012. Retinoic Acid Induces Apoptosis of Prostate Cancer DU145 Cells through Cdk5 Overactivation. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-1028403
Modern Language Association (MLA)
Chen, Mei-Chih…[et al.]. Retinoic Acid Induces Apoptosis of Prostate Cancer DU145 Cells through Cdk5 Overactivation. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-1028403
American Medical Association (AMA)
Chen, Mei-Chih& Huang, Chih-Yang& Hsu, Shih-Lan& Lin, Eugene& Ku, Chien-Te& Lin, Ho…[et al.]. Retinoic Acid Induces Apoptosis of Prostate Cancer DU145 Cells through Cdk5 Overactivation. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-1028403
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1028403