Chloroform Fraction of Centratherum anthelminticum (L.) Seed Inhibits Tumor Necrosis Factor Alpha and Exhibits Pleotropic Bioactivities: Inhibitory Role in Human Tumor Cells
Joint Authors
Arya, Aditya
Achoui, Mouna
Cheah, Shiau-Chuen
Narrima, Putri
Mustafa, Mohd Rais
Abdel Wahab, Siddig Ibrahim
Mohan, Syam
Rais Mustafa, Mohd
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-28
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
We investigated the antioxidant potential, cytotoxic effect, and TNF-α inhibition activity with NF-κB activation response in a chloroform fraction of Centratherum anthelminticum seeds (CACF).
The antioxidant property of CACF was evaluated with DPPH, ORAC, and FRAP assays, which demonstrated significant antioxidant activity.
The cytotoxicity of CACF was tested using the MTT assay; CACF effective inhibitory concentrations (IC50) for A549, PC-3, MCF-7, and WRL-68 cells were 31.42±5.4, 22.61±1.7, 8.1±0.9, and 54.93±8.3 μg/mL, respectively.
CACF effectively and dose-dependently inhibited TNF-α release, in vitro and in vivo.
CACF inhibited TNF-α secretion in stimulated RAW264.7 macrophage supernatants with an IC50 of 0.012 μg/mL, without affecting their viability; the highest dose tested reduced serum TNF-α by 61%.
Acute toxicity testing in rats revealed that CACF was non-toxic at all doses tested.
Matching the cytotoxic activity towards a mechanistic approach, CACF dose-dependently exhibited in vitro inhibitory effects against the activation of NF-κB translocation in MCF-7 cells.
Preliminary phytochemical screening with GC/MS analysis detected 22 compounds in CACF, of which morpholinoethyl isothiocyanate was the most abundant (29.04%).
The study reveals the potential of CACF in the treatment of breast cancer and in oxidative stress conditions with associated inflammatory responses.
American Psychological Association (APA)
Arya, Aditya& Achoui, Mouna& Cheah, Shiau-Chuen& Abdel Wahab, Siddig Ibrahim& Narrima, Putri& Mohan, Syam…[et al.]. 2012. Chloroform Fraction of Centratherum anthelminticum (L.) Seed Inhibits Tumor Necrosis Factor Alpha and Exhibits Pleotropic Bioactivities: Inhibitory Role in Human Tumor Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-1028437
Modern Language Association (MLA)
Arya, Aditya…[et al.]. Chloroform Fraction of Centratherum anthelminticum (L.) Seed Inhibits Tumor Necrosis Factor Alpha and Exhibits Pleotropic Bioactivities: Inhibitory Role in Human Tumor Cells. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-1028437
American Medical Association (AMA)
Arya, Aditya& Achoui, Mouna& Cheah, Shiau-Chuen& Abdel Wahab, Siddig Ibrahim& Narrima, Putri& Mohan, Syam…[et al.]. Chloroform Fraction of Centratherum anthelminticum (L.) Seed Inhibits Tumor Necrosis Factor Alpha and Exhibits Pleotropic Bioactivities: Inhibitory Role in Human Tumor Cells. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-1028437
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1028437