Attenuation of TRPV1 and TRPV4 Expression and Function in Mouse Inflammatory Pain Models Using Electroacupuncture
Joint Authors
Lin, Yi-Wen
Lin, Tzu-Jou
Chen, Shih-Yin
Chen, Yung-Hsiang
Tzen, Jason T. C.
Hsieh, Ching-Liang
Chen, Wei-Hsin
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-13
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Although pain is a major human affliction, our understanding of pain mechanisms is limited.
TRPV1 (transient receptor potential vanilloid subtype 1) and TRPV4 are two crucial receptors involved in inflammatory pain, but their roles in EA- (electroacupuncture-) mediated analgesia are unknown.
We injected mice with carrageenan (carra) or a complete Freund’s adjuvant (CFA) to model inflammatory pain and investigated the analgesic effect of EA using animal behavior tests, immunostaining, Western blotting, and a whole-cell recording technique.
The inflammatory pain model mice developed both mechanical and thermal hyperalgesia.
Notably, EA at the ST36 acupoint reversed these phenomena, indicating its curative effect in inflammatory pain.
The protein levels of TRPV1 and TRPV4 in DRG (dorsal root ganglion) neurons were both increased at day 4 after the initiation of inflammatory pain and were attenuated by EA, as demonstrated by immunostaining and Western blot analysis.
We verified DRG electrophysiological properties to confirm that EA ameliorated peripheral nerve hyperexcitation.
Our results indicated that the AP (action potential) threshold, rise time, and fall time, and the percentage and amplitude of TRPV1 and TRPV4 were altered by EA, indicating that EA has an antinociceptive role in inflammatory pain.
Our results demonstrate a novel role for EA in regulating TRPV1 and TRPV4 protein expression and nerve excitation in mouse inflammatory pain models.
American Psychological Association (APA)
Chen, Wei-Hsin& Tzen, Jason T. C.& Hsieh, Ching-Liang& Chen, Yung-Hsiang& Lin, Tzu-Jou& Chen, Shih-Yin…[et al.]. 2012. Attenuation of TRPV1 and TRPV4 Expression and Function in Mouse Inflammatory Pain Models Using Electroacupuncture. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-1028444
Modern Language Association (MLA)
Chen, Wei-Hsin…[et al.]. Attenuation of TRPV1 and TRPV4 Expression and Function in Mouse Inflammatory Pain Models Using Electroacupuncture. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-1028444
American Medical Association (AMA)
Chen, Wei-Hsin& Tzen, Jason T. C.& Hsieh, Ching-Liang& Chen, Yung-Hsiang& Lin, Tzu-Jou& Chen, Shih-Yin…[et al.]. Attenuation of TRPV1 and TRPV4 Expression and Function in Mouse Inflammatory Pain Models Using Electroacupuncture. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-1028444
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1028444