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Electroacupuncture Confers Antinociceptive Effects via Inhibition of Glutamate Transporter Downregulation in Complete Freund's Adjuvant-Injected Rats
Joint Authors
Choi, Byung Tae
Kim, Ha-Neui
Jang, Ji-Yeon
Kim, Yu-Ri
Shin, Hwa Kyoung
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-08-08
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
When we evaluated changes of glial fibrillary acidic protein (GFAP) and two glutamate transporter (GTs) by immunohistochemistry, expression of GFAP showed a significant increase in complete Freund's adjuvant (CFA)-injected rats; however, this expression was strongly inhibited by electroacupuncture (EA) stimulation.
Robust downregulation of glutamate-aspartate transporter (GLAST) and glutamate transporter-1 (GLT-1) was observed in CFA-injected rats; however, EA stimulation resulted in recovery of this expression.
Double-labeling staining showed co-localization of a large proportion of GLAST or GLT-1 with GFAP.
Using Western blot, we confirmed protein expression of two GTs, but no differences in the mRNA content of these GTs were observed.
Because EA treatment resulted in strong inhibition of CFA-induced proteasome activities, we examined the question of whether thermal sensitivities and GTs expression could be regulated by proteasome inhibitor MG132.
CFA-injected rats co-treated with EA and MG132 showed a significantly longer thermal sensitivity, compared with CFA-injected rats with or without MG132.
Both EA and MG132 blocked CFA-induced GLAST and GLT-1 downregulation within the spinal cord.
These results provide evidence for involvement of GLAST and GLT-1 in response to activation of spinal astrocytes in an EA antinociceptive effect.
Antinociceptive effect of EA may be induced via proteasome-mediated regulation of spinal GTs.
American Psychological Association (APA)
Kim, Ha-Neui& Kim, Yu-Ri& Jang, Ji-Yeon& Shin, Hwa Kyoung& Choi, Byung Tae. 2012. Electroacupuncture Confers Antinociceptive Effects via Inhibition of Glutamate Transporter Downregulation in Complete Freund's Adjuvant-Injected Rats. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-1028449
Modern Language Association (MLA)
Kim, Ha-Neui…[et al.]. Electroacupuncture Confers Antinociceptive Effects via Inhibition of Glutamate Transporter Downregulation in Complete Freund's Adjuvant-Injected Rats. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-1028449
American Medical Association (AMA)
Kim, Ha-Neui& Kim, Yu-Ri& Jang, Ji-Yeon& Shin, Hwa Kyoung& Choi, Byung Tae. Electroacupuncture Confers Antinociceptive Effects via Inhibition of Glutamate Transporter Downregulation in Complete Freund's Adjuvant-Injected Rats. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-1028449
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1028449