The Antiproliferative Activity of Sclerotia of Lignosus rhinocerus (Tiger Milk Mushroom)
Joint Authors
Fung, Shin Yee
Tan, Nget Hong
Lee, M. L.
Tan, C. S.
Ng, S. T.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-13
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Lignosus rhinocerus, the tiger milk mushroom, is one of the most important medicinal mushrooms used by the indigenous people of Southeast Asia and China.
It has been used to treat breast cancer.
A cold water extract (LR-CW) prepared from the sclerotia of L.
rhinocerus cultivar was found to exhibit antiproliferative activity against human breast carcinoma (MCF-7) and human lung carcinoma (A549), with IC50 of 96.7 μg/mL and 466.7 μg/mL, respectively.
In comparison, LR-CW did not show significant cytotoxicity against the two corresponding human normal cells, 184B5 (human breast cell) and NL 20 (human lung cell).
DNA fragmentation studies suggested that the cytotoxic action of LR-CW against cancer cells is mediated by apoptosis.
Sephadex G-50 gel filtration fractionation of LR-CW yielded a high-molecular-weight and a low-molecular-weight fraction.
The high-molecular-weight fraction contains mainly carbohydrate (68.7%) and small amount of protein (3.6%), whereas the low-molecular-weight fraction contains 31% carbohydrate and was devoid of protein.
Only the high-molecular-weight fraction exhibited antiproliferative activity against cancer cells, with IC50 of 70.0 μg/mL and 76.7 μg/mL, respectively.
Thus, the cytotoxic action of the LR-CW is due to the high-molecular-weight fraction, either the proteins or protein-carbohydrate complex.
American Psychological Association (APA)
Lee, M. L.& Tan, Nget Hong& Fung, Shin Yee& Tan, C. S.& Ng, S. T.. 2012. The Antiproliferative Activity of Sclerotia of Lignosus rhinocerus (Tiger Milk Mushroom). Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-1028482
Modern Language Association (MLA)
Lee, M. L.…[et al.]. The Antiproliferative Activity of Sclerotia of Lignosus rhinocerus (Tiger Milk Mushroom). Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-1028482
American Medical Association (AMA)
Lee, M. L.& Tan, Nget Hong& Fung, Shin Yee& Tan, C. S.& Ng, S. T.. The Antiproliferative Activity of Sclerotia of Lignosus rhinocerus (Tiger Milk Mushroom). Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-1028482
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1028482