Jaceosidin Induces Apoptosis in U87 Glioblastoma Cells through G2M Phase Arrest
Joint Authors
Rasul, Azhar
Khan, Muhammad
Yu, Bo
Al Shawi, Ali
Yi, Fei
Yang, Hong
Ma, Tonghui
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-19
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Artemisia argyi is a widely used medicinal plant in China.
The present study was designed to identify the bioactive constituents with antiglioma activity from leaves of Artemesia argyi.
A bioactivity guided approach based on MTT assay for cells growth inhibition led to the isolation of a flavonoid, “jaceosidin” from ethanol extract of leaves of Artemesia argyi.
The growth inhibitory effect of jaceosidin was explored using flow cytometry and Western blot studies.
Our results showed that jaceosidin exerts growth inhibitory effect by arresting the cells at G2/M phase and induction of apoptosis.
Furthermore, our study revealed that induction of apoptosis was associated with cell cycle arrest at G2/M phase, upregulation of p53 and Bax, decrease in mitochondrial membrane potential, release of cytochrome c, and activation of caspase 3.
This mitochondrial-caspase-3-dependent apoptosis pathway was confirmed by pretreatment with caspase 3 inhibitor, Ac-DEVD-CHO.
Our findings suggested that jaceosidin induces mitochondrial-caspase-3-dependent apoptosis in U87 cells by arresting the cell cycle at G2/M phase.
American Psychological Association (APA)
Khan, Muhammad& Yu, Bo& Rasul, Azhar& Al Shawi, Ali& Yi, Fei& Yang, Hong…[et al.]. 2011. Jaceosidin Induces Apoptosis in U87 Glioblastoma Cells through G2M Phase Arrest. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-1028488
Modern Language Association (MLA)
Khan, Muhammad…[et al.]. Jaceosidin Induces Apoptosis in U87 Glioblastoma Cells through G2M Phase Arrest. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-1028488
American Medical Association (AMA)
Khan, Muhammad& Yu, Bo& Rasul, Azhar& Al Shawi, Ali& Yi, Fei& Yang, Hong…[et al.]. Jaceosidin Induces Apoptosis in U87 Glioblastoma Cells through G2M Phase Arrest. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-1028488
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1028488