Phenethyl Isothiocyanate (PEITC) Inhibits the Growth of Human Oral Squamous Carcinoma HSC-3 Cells through G 0 G 1 Phase Arrest and Mitochondria-Mediated Apoptotic Cell Death
Joint Authors
Chung, Jing-Gung
Chen, Po-Yuan
Lin, Kai-Chun
Tang, Nou-Ying
Lu, Kung-Wen
Yang, Jai-Sing
Lin, Jing-Pin
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-10
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Phenethyl isothiocyanate (PEITC), an effective anticancer and chemopreventive agent, has been reported to inhibit cancer cell growth through cell-cycle arrest and induction of apoptotic events in various human cancer cells models.
However, whether PEITC inhibits human oral squamous cell carcinoma HSC-3 cell growth and its underlying mechanisms is still not well elucidated.
In the present study, we evaluated the inhibitory effects of PEITC in HSC-3 cells and examined PEITC-modulated cell-cycle arrest and apoptosis.
The contrast-phase and flow cytometric assays were used for examining cell morphological changes and viability, respectively.
The changes of cell-cycle and apoptosis-associated protein levels were determined utilizing Western blotting in HSC-3 cells after exposure to PEITC.
Our results indicated that PEITC effectively inhibited the HSC-3 cells’ growth and caused apoptosis.
PEITC induced G 0 / G 1 phase arrest through the effects of associated protein such as p53, p21, p17, CDK2 and cyclin E, and it triggered apoptosis through promotion of Bax and Bid expression and reduction of Bcl-2, leading to decrease the levels of mitochondrial membrane potential ( Δ Ψ m ), and followed the releases of cytochrome c, AIF and Endo G then for causing apoptosis in HSC-3 cells.
These results suggest that PEITC could be an antitumor compound for oral cancer therapy.
American Psychological Association (APA)
Chen, Po-Yuan& Lin, Kai-Chun& Lin, Jing-Pin& Tang, Nou-Ying& Yang, Jai-Sing& Lu, Kung-Wen…[et al.]. 2012. Phenethyl Isothiocyanate (PEITC) Inhibits the Growth of Human Oral Squamous Carcinoma HSC-3 Cells through G 0 G 1 Phase Arrest and Mitochondria-Mediated Apoptotic Cell Death. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-1028503
Modern Language Association (MLA)
Chen, Po-Yuan…[et al.]. Phenethyl Isothiocyanate (PEITC) Inhibits the Growth of Human Oral Squamous Carcinoma HSC-3 Cells through G 0 G 1 Phase Arrest and Mitochondria-Mediated Apoptotic Cell Death. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-1028503
American Medical Association (AMA)
Chen, Po-Yuan& Lin, Kai-Chun& Lin, Jing-Pin& Tang, Nou-Ying& Yang, Jai-Sing& Lu, Kung-Wen…[et al.]. Phenethyl Isothiocyanate (PEITC) Inhibits the Growth of Human Oral Squamous Carcinoma HSC-3 Cells through G 0 G 1 Phase Arrest and Mitochondria-Mediated Apoptotic Cell Death. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-1028503
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1028503