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Terpinen-4-ol Induces Apoptosis in Human Nonsmall Cell Lung Cancer In Vitro and In Vivo
Joint Authors
Chen, Jeremy J. W.
Huang, Chia-Hsin
Lin, Pei-Shan
Chang, Gee-Chen
Chang, JingHua-Tsai
Wu, Chieh-Shan
Shieh, Jeng-Jer
Lin, Chi-Chen
Chen, Yun-Ju
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-31
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Terpinen-4-ol, a monoterpene component of the essential oils of several aromatic plants, exhibits antitumor effects.
In this study, the antitumor effects of terpinen-4-ol and the cellular and molecular mechanisms responsible for it were evaluated and studied, respectively on human nonsmall cell lung cancer (NSCLC) cells.
Our results indicated that terpinen-4-ol elicited a dose-dependent cytotoxic effect, as determined by MTT assay.
Increased sub-G1 population and annexin-V binding, activation of caspases 9 and 3, cleavage of poly(ADPribose) polymerase (PARP), and a decrease of mitochondrial membrane potential (MMP) indicated involvement of the mitochondrial apoptotic pathway in terpinen-4-ol-treated A549 and CL1-0 cells.
Elevation of the Bax/Bcl-2 ratio and a decrease in IAP family proteins XIAP and survivin were also observed following terpinen-4-ol treatment.
Notably, terpinen-4-ol was able to increase p53 levels in A549 and CL1-0 cells.
Diminution of p53 by RNA interference induced necrosis instead of apoptosis in A549 cells following terpinen-4-ol treatment, indicating that terpinen-4-ol-elicited apoptosis is p53-dependent.
Moreover, intratumoral administration of terpinen-4-ol significantly suppressed the growth of s.c.
A549 xenografts by inducing apoptosis, as confirmed by TUNEL assay.
Collectively, these data provide insight into the molecular mechanisms underlying terpinen-4-ol-induced apoptosis in NSCLC cells, rendering this compound a potential anticancer drug for NSCLC.
American Psychological Association (APA)
Wu, Chieh-Shan& Chen, Yun-Ju& Chen, Jeremy J. W.& Shieh, Jeng-Jer& Huang, Chia-Hsin& Lin, Pei-Shan…[et al.]. 2011. Terpinen-4-ol Induces Apoptosis in Human Nonsmall Cell Lung Cancer In Vitro and In Vivo. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-1028559
Modern Language Association (MLA)
Wu, Chieh-Shan…[et al.]. Terpinen-4-ol Induces Apoptosis in Human Nonsmall Cell Lung Cancer In Vitro and In Vivo. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-13.
https://search.emarefa.net/detail/BIM-1028559
American Medical Association (AMA)
Wu, Chieh-Shan& Chen, Yun-Ju& Chen, Jeremy J. W.& Shieh, Jeng-Jer& Huang, Chia-Hsin& Lin, Pei-Shan…[et al.]. Terpinen-4-ol Induces Apoptosis in Human Nonsmall Cell Lung Cancer In Vitro and In Vivo. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-1028559
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1028559