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Antimicrobial Effects of a Lipophilic Fraction and Kaurenoic Acid Isolated from the Root Bark Extracts of Annona senegalensis
Joint Authors
Okoye, Theophine Chinwuba
Okoli, Charles Ogbonnaya
Ezike, Adaobi Chioma
Omeje, Edwin Ogechukwu
Odoh, Uchenna Estella
Akah, Peter A.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-24
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Root bark preparation of Annona senegalensis Pers.
(Annonaceae) is used in Nigerian ethnomedicine for treatment of infectious diseases.
Extraction of the A.
senegalensis powdered root bark with methanol-methylene chloride (1 : 1) mixture yielded the methanol-methylene extract (MME) which was fractionated to obtain the ethyl acetate fraction (EF).
The EF on further fractionation gave two active subfractions, F1 and F2.
The F1 yielded a lipophilic oily liquid while F2 on purification, precipitated white crystalline compound, AS2.
F1 was analyzed using GC-MS, while AS2 was characterized by proton NMR and X-ray crystallography.
Antibacterial and antifungal studies were performed using agar-well-diffusion method with 0.5 McFarland standard and MICs calculated.
GC-MS gave 6 major constituents: kaur-16-en-19-oic acid; 1-dodecanol; 1-naphthalenemethanol; 6,6-dimethyl-bicyclo[3.1.1]hept-2-ene-2-ethanol; 3,3-dimethyl-2-(3-methylbuta-1,3-dienyl)cyclohexane-1-methanol; 3-hydroxyandrostan-17-carboxylic acid.
AS2 was found to be kaur-16-en-19-oic acid.
The MICs of EF, F1, and AS2 against B.
subtilis were 180, 60, and 30 μg/mL, respectively.
AS2 exhibited activity against S.
aureus with an MIC of 150 μg/mL, while F1 was active against P.
aeruginosa with an MIC of 40 μg/mL.
However, the extracts and AS2 exhibited no effects against Candida albicans and Aspergillus niger.
Therefore, kaurenoic acid and the lipophilic fraction from A.
senegalensis root bark exhibited potent antibacterial activity.
American Psychological Association (APA)
Okoye, Theophine Chinwuba& Akah, Peter A.& Okoli, Charles Ogbonnaya& Ezike, Adaobi Chioma& Omeje, Edwin Ogechukwu& Odoh, Uchenna Estella. 2012. Antimicrobial Effects of a Lipophilic Fraction and Kaurenoic Acid Isolated from the Root Bark Extracts of Annona senegalensis. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-1028570
Modern Language Association (MLA)
Okoye, Theophine Chinwuba…[et al.]. Antimicrobial Effects of a Lipophilic Fraction and Kaurenoic Acid Isolated from the Root Bark Extracts of Annona senegalensis. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-1028570
American Medical Association (AMA)
Okoye, Theophine Chinwuba& Akah, Peter A.& Okoli, Charles Ogbonnaya& Ezike, Adaobi Chioma& Omeje, Edwin Ogechukwu& Odoh, Uchenna Estella. Antimicrobial Effects of a Lipophilic Fraction and Kaurenoic Acid Isolated from the Root Bark Extracts of Annona senegalensis. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-1028570
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1028570