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Dentatin Induces Apoptosis in Prostate Cancer Cells via Bcl-2, Bcl-xL, Survivin Downregulation, Caspase-9, -37 Activation, and NF-κB Inhibition
Joint Authors
Mohamed Elhassan Taha, Manal
Arya, Aditya
Looi, Chung Yeng
Wong, Won Fen
Abdul, Ahmad Bustamam
Cheah, Foo Kit
Abdullah, Rasedee
Muharram, Bushra
Ibrahim Abdelwahab, Siddig
Syam, Suvitha
Arbab, Ismail Adam
Mohan, Syam
Sukari, Mohd Aspollah
Rais Mustafa, Mohd
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-03
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
This study was set to investigate antiproliferative potential of dentatin (a natural coumarin isolated from Clausena excavata Burm.
F) against prostate cancer and to delineate the underlying mechanism of action.
Treatment with dentatin dose-dependently inhibited cell growth of PC-3 and LNCaP prostate cancer cell lines, whereas it showed less cytotoxic effects on normal prostate epithelial cell line (RWPE-1).
The inhibitory effect of dentatin on prostate cancer cell growth was due to induction of apoptosis as evidenced by Annexin V staining and cell shrinkage.
We found that dentatin-mediated accumulation of reactive oxygen species (ROS) and downregulated expression levels of antiapoptotic molecules (Bcl-2, Bcl-xL, and Survivin), leading to disruption of mitochondrial membrane potential (MMP), cell membrane permeability, and release of cytochrome c from the mitochondria into the cytosol.
These effects were associated with induction of caspase-9, -3/7 activities, and subsequent DNA fragmentation.
In addition, we found that dentatin inhibited TNF-α-induced nuclear translocation of p65, suggesting dentatin as a potential NF-κB inhibitor.
Thus, we suggest that dentatin may have therapeutic value in prostate cancer treatment worthy of further development.
American Psychological Association (APA)
Arbab, Ismail Adam& Looi, Chung Yeng& Abdul, Ahmad Bustamam& Cheah, Foo Kit& Wong, Won Fen& Sukari, Mohd Aspollah…[et al.]. 2012. Dentatin Induces Apoptosis in Prostate Cancer Cells via Bcl-2, Bcl-xL, Survivin Downregulation, Caspase-9, -37 Activation, and NF-κB Inhibition. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-15.
https://search.emarefa.net/detail/BIM-1028586
Modern Language Association (MLA)
Arbab, Ismail Adam…[et al.]. Dentatin Induces Apoptosis in Prostate Cancer Cells via Bcl-2, Bcl-xL, Survivin Downregulation, Caspase-9, -37 Activation, and NF-κB Inhibition. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-15.
https://search.emarefa.net/detail/BIM-1028586
American Medical Association (AMA)
Arbab, Ismail Adam& Looi, Chung Yeng& Abdul, Ahmad Bustamam& Cheah, Foo Kit& Wong, Won Fen& Sukari, Mohd Aspollah…[et al.]. Dentatin Induces Apoptosis in Prostate Cancer Cells via Bcl-2, Bcl-xL, Survivin Downregulation, Caspase-9, -37 Activation, and NF-κB Inhibition. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-15.
https://search.emarefa.net/detail/BIM-1028586
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1028586