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Bioguided Fractionation Shows Cassia alata Extract to Inhibit Staphylococcus epidermidis and Pseudomonas aeruginosa Growth and Biofilm Formation
Joint Authors
Gosmann, Grace
Saito, Samuel Takashi
Trentin, Danielle da Silva
Pungartnik, Cristina
Silveira, Jaqueline de Deos
Guecheva, Temenouga Nikolova
Henriques, João Antonio Pêgas
Brendel, Martin
Macedo, Alexandre José
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-02
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Plant extracts have a long history to be used in folk medicine.
Cassia alata extracts are known to exert antibacterial activity but details on compounds and mechanism of action remain poorly explored.
We purified and concentrated the aqueous leaf extract of C.
alata by reverse phase-solid phase extraction and screened the resulting CaRP extract for antimicrobial activity.
CaRP extract exhibited antimicrobial activity for Pseudomonas aeruginosa, Staphylococcus epidermidis, S.
aureus, and Bacillus subtilis.
CaRP also inhibited biofilm formation of S.
epidermidis and P.
aeruginosa.
Several bacterial growth-inhibiting compounds were detected when CaRP extract was fractionated by TLC chromatography coupled to bioautography agar overlay technique.
HPLC chromatography of CaRP extract yielded 20 subfractions that were tested by bioautography for antimicrobial activity against S.
aureus and S.
epidermidis.
Five bioactive fractions were detected and chemically characterized, using high-resolution mass spectrometry (qTOF-MS/MS).
Six compounds from four fractions could be characterized as kaempferol, kaempferol-O-diglucoside, kaempferol-O-glucoside, quercetin-O-glucoside, rhein, and danthron.
In the Salmonella/microsome assay CaRP showed weak mutagenicity (MI<3) only in strain TA98, pointing to a frameshift mutation activity.
These results indicate that C.
alata leaf extract contains a minimum of 7 compounds with antimicrobial activity and that these together or as single substance are active in preventing formation of bacterial biofilm, indicating potential for therapeutic applications.
American Psychological Association (APA)
Saito, Samuel Takashi& Trentin, Danielle da Silva& Macedo, Alexandre José& Pungartnik, Cristina& Gosmann, Grace& Silveira, Jaqueline de Deos…[et al.]. 2012. Bioguided Fractionation Shows Cassia alata Extract to Inhibit Staphylococcus epidermidis and Pseudomonas aeruginosa Growth and Biofilm Formation. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-1028593
Modern Language Association (MLA)
Saito, Samuel Takashi…[et al.]. Bioguided Fractionation Shows Cassia alata Extract to Inhibit Staphylococcus epidermidis and Pseudomonas aeruginosa Growth and Biofilm Formation. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-13.
https://search.emarefa.net/detail/BIM-1028593
American Medical Association (AMA)
Saito, Samuel Takashi& Trentin, Danielle da Silva& Macedo, Alexandre José& Pungartnik, Cristina& Gosmann, Grace& Silveira, Jaqueline de Deos…[et al.]. Bioguided Fractionation Shows Cassia alata Extract to Inhibit Staphylococcus epidermidis and Pseudomonas aeruginosa Growth and Biofilm Formation. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-1028593
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1028593