Expression of Endoplasmic Reticulum Stress-Related Factors in the Retinas of Diabetic Rats
Joint Authors
Hu, Wei-kun
Zheng, Cui
Chen, Zhi-qi
Yan, Shu
Li, Bin
Li, Gui-gang
Liu, Rong
Pei, Han
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-08-04
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Recent reports show that ER stress plays an important role in diabetic retinopathy (DR), but ER stress is a complicated process involving a network of signaling pathways and hundreds of factors, What factors involved in DR are not yet understood.
We selected 89 ER stress factors from more than 200, A rat diabetes model was established by intraperitoneal injection of streptozotocin (STZ).
The expression of 89 ER stress-related factors was found in the retinas of diabetic rats, at both 1- and 3-months after development of diabetes, by quantitative real-time polymerase chain reaction arrays.
There were significant changes in expression levels of 13 and 12 ER stress-related factors in the diabetic rat retinas in the first and third month after the development of diabetes, Based on the array results, homocysteine- inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1(HERP), and synoviolin(HRD1) were studied further by immunofluorescence and Western blot.
Immunofluorescence and Western blot analyses showed that the expression of HERP was reduced in the retinas of diabetic rats in first and third month.
The expression of Hrd1 did not change significantly in the retinas of diabetic rats in the first month but was reduced in the third month.
American Psychological Association (APA)
Yan, Shu& Zheng, Cui& Chen, Zhi-qi& Liu, Rong& Li, Gui-gang& Hu, Wei-kun…[et al.]. 2011. Expression of Endoplasmic Reticulum Stress-Related Factors in the Retinas of Diabetic Rats. Journal of Diabetes Research،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-1029086
Modern Language Association (MLA)
Yan, Shu…[et al.]. Expression of Endoplasmic Reticulum Stress-Related Factors in the Retinas of Diabetic Rats. Journal of Diabetes Research Vol. 2012, no. 2012 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-1029086
American Medical Association (AMA)
Yan, Shu& Zheng, Cui& Chen, Zhi-qi& Liu, Rong& Li, Gui-gang& Hu, Wei-kun…[et al.]. Expression of Endoplasmic Reticulum Stress-Related Factors in the Retinas of Diabetic Rats. Journal of Diabetes Research. 2011. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-1029086
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1029086