Pentoxifylline Attenuates Methionine- and Choline-Deficient-Diet-Induced Steatohepatitis by Suppressing TNF-α Expression and Endoplasmic Reticulum Stress

Joint Authors

Chae, Min Kyung
Lee, Byung-Wan
Cha, Bong Soo
Lee, Hyun Chul
Park, Sang Gyu
Song, Sun-Ok
Kang, Eun Seok

Source

Journal of Diabetes Research

Issue

Vol. 2012, Issue 2012 (31 Dec. 2011), pp.1-8, 8 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2012-01-29

Country of Publication

Egypt

No. of Pages

8

Main Subjects

Diseases
Medicine

Abstract EN

Background.

Pentoxifylline (PTX) anti-TNF properties are known to exert hepatoprotective effects in various liver injury models.

The aim of this study was to investigate whether PTX has beneficial roles in the development of methionine- and choline-deficient-(MCD-) diet-induced NAFLD SD rats in vivo and TNF-α-induced Hep3B cells in vitro.

Methods.

SD Rats were classified according to diet (chow or MCD diet) and treatment (normal saline or PTX injection) over a period of 4 weeks: group I (chow + saline, n=4), group II (chow + PTX), group III (MCD + saline), and group IV (MCD + PTX).

Hep3B cells were treated with 100 ng/ml TNF-α (24 h) in the absence or presence of PTX (1 mM).

Results.

PTX attenuated MCD-diet-induced serum ALT levels and hepatic steatosis.

In real-time PCR and western blotting analysis, PTX decreased MCD-diet-induced TNF-alpha mRNA expression and proapoptotic unfolded protein response by ER stress (GRP78, p-eIF2, ATF4, IRE1α, CHOP, and p-JNK activation) in vivo.

PTX (1 mM) reduced TNF-α-induced activation of GRP78, p-eIF2, ATF4, IRE1α, and CHOP in vitro.

Conclusion.

PTX has beneficial roles in the development of MCD-diet-induced steatohepatitis through partial suppression of TNF-α and ER stress.

American Psychological Association (APA)

Chae, Min Kyung& Park, Sang Gyu& Song, Sun-Ok& Kang, Eun Seok& Cha, Bong Soo& Lee, Hyun Chul…[et al.]. 2012. Pentoxifylline Attenuates Methionine- and Choline-Deficient-Diet-Induced Steatohepatitis by Suppressing TNF-α Expression and Endoplasmic Reticulum Stress. Journal of Diabetes Research،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-1029089

Modern Language Association (MLA)

Chae, Min Kyung…[et al.]. Pentoxifylline Attenuates Methionine- and Choline-Deficient-Diet-Induced Steatohepatitis by Suppressing TNF-α Expression and Endoplasmic Reticulum Stress. Journal of Diabetes Research Vol. 2012, no. 2012 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-1029089

American Medical Association (AMA)

Chae, Min Kyung& Park, Sang Gyu& Song, Sun-Ok& Kang, Eun Seok& Cha, Bong Soo& Lee, Hyun Chul…[et al.]. Pentoxifylline Attenuates Methionine- and Choline-Deficient-Diet-Induced Steatohepatitis by Suppressing TNF-α Expression and Endoplasmic Reticulum Stress. Journal of Diabetes Research. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-1029089

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1029089