Toll-Like Receptor Transcriptome in the HPV-Positive Cervical Cancer Microenvironment
Joint Authors
Zehbe, Ingeborg
Jackson, Robert
Escott, Nicholas G.
Rosa, Bruce
Niccoli, Sarah
DeCarlo, Correne A.
Source
Journal of Immunology Research
Issue
Vol. 2012, Issue 2012 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-13
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The human papillomavirus (HPV) directly infects cervical keratinocytes and interferes with TLR signalling.
To shed light on the effect of HPV on upstream receptors, we evaluated TLRs 1–9 gene expression in HPV-negative normal and HPV-positive pre-malignant and malignant ex vivo cervical tissue.
Quantitative real-time polymerase chain reaction was performed separately for epithelial and stromal tissue compartments.
Differences in gene expression were analyzed by the Jonckheere-Terpstra trend test or the Student's t-test for pairwise comparison.
Laser capture microdissection revealed an increase in TLR3 and a decrease in TLR1 mRNA levels in dysplastic and carcinoma epithelium, respectively.
In the stroma, a trend of increasing TLR 1, 2, 5, 6, and 9 mRNA levels with disease severity was found.
These findings implicate the involvement of TLR3 and TLR1 in early and late cervical carcinogenesis, respectively, suggesting that stromal upregulation of TLRs may play a role in cervical disease progression.
American Psychological Association (APA)
DeCarlo, Correne A.& Rosa, Bruce& Jackson, Robert& Niccoli, Sarah& Escott, Nicholas G.& Zehbe, Ingeborg. 2011. Toll-Like Receptor Transcriptome in the HPV-Positive Cervical Cancer Microenvironment. Journal of Immunology Research،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-1029115
Modern Language Association (MLA)
DeCarlo, Correne A.…[et al.]. Toll-Like Receptor Transcriptome in the HPV-Positive Cervical Cancer Microenvironment. Journal of Immunology Research Vol. 2012, no. 2012 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-1029115
American Medical Association (AMA)
DeCarlo, Correne A.& Rosa, Bruce& Jackson, Robert& Niccoli, Sarah& Escott, Nicholas G.& Zehbe, Ingeborg. Toll-Like Receptor Transcriptome in the HPV-Positive Cervical Cancer Microenvironment. Journal of Immunology Research. 2011. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-1029115
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1029115