scFv Antibody: Principles and Clinical Application
Joint Authors
Alitheen, Noorjahan Banu Mohamed
Ahmad, Zuhaida Asra
Hamid, Muhajir
Ali, Abdul Manaf
Ho, Wan Yong
Yeap, Swee Keong
Source
Journal of Immunology Research
Issue
Vol. 2012, Issue 2012 (31 Dec. 2011), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-15
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
To date, generation of single-chain fragment variable (scFv) has become an established technique used to produce a completely functional antigen-binding fragment in bacterial systems.
The advances in antibody engineering have now facilitated a more efficient and generally applicable method to produce Fv fragments.
Basically, scFv antibodies produced from phage display can be genetically fused to the marker proteins, such as fluorescent proteins or alkaline phosphatase.
These bifunctional proteins having both antigen-binding capacity and marker activity can be obtained from transformed bacteria and used for one-step immunodetection of biological agents.
Alternatively, antibody fragments could also be applied in the construction of immunotoxins, therapeutic gene delivery, and anticancer intrabodies for therapeutic purposes.
This paper provides an overview of the current studies on the principle, generation, and application of scFv.
The potential of scFv in breast cancer research is also discussed in this paper.
American Psychological Association (APA)
Ahmad, Zuhaida Asra& Yeap, Swee Keong& Ali, Abdul Manaf& Ho, Wan Yong& Alitheen, Noorjahan Banu Mohamed& Hamid, Muhajir. 2012. scFv Antibody: Principles and Clinical Application. Journal of Immunology Research،Vol. 2012, no. 2012, pp.1-15.
https://search.emarefa.net/detail/BIM-1029123
Modern Language Association (MLA)
Ahmad, Zuhaida Asra…[et al.]. scFv Antibody: Principles and Clinical Application. Journal of Immunology Research Vol. 2012, no. 2012 (2011), pp.1-15.
https://search.emarefa.net/detail/BIM-1029123
American Medical Association (AMA)
Ahmad, Zuhaida Asra& Yeap, Swee Keong& Ali, Abdul Manaf& Ho, Wan Yong& Alitheen, Noorjahan Banu Mohamed& Hamid, Muhajir. scFv Antibody: Principles and Clinical Application. Journal of Immunology Research. 2012. Vol. 2012, no. 2012, pp.1-15.
https://search.emarefa.net/detail/BIM-1029123
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1029123