Gold Nanoparticle Sensor for the Visual Detection of Pork Adulteration in Meatball Formulation
Joint Authors
Mustafa, Shuhaimi
Islam, Kh. Nurul
Hashim, U.
Ali, M. E.
Che Man, Y. B.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-30
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
We visually identify pork adulteration in beef and chicken meatball preparations using 20 nm gold nanoparticles (GNPs) as colorimetric sensors.
Meatball is a popular food in certain Asian and European countries.
Verification of pork adulteration in meatball is necessary to meet the Halal and Kosher food standards.
Twenty nm GNPs change color from pinkish-red to gray-purple, and their absorption peak at 525 nm is red-shifted by 30–50 nm in 3 mM phosphate buffer saline (PBS).
Adsorption of single-stranded DNA protects the particles against salt-induced aggregation.
Mixing and annealing of a 25-nucleotide (nt) single-stranded (ss) DNA probe with denatured DNA of different meatballs differentiated well between perfectly matched and mismatch hybridization at a critical annealing temperature.
The probes become available in nonpork DNA containing vials due to mismatches and interact with GNPs to protect them from salt-induced aggregation.
Whereas, all the pork containing vials, either in pure and mixed forms, consumed the probes totally by perfect hybridization and turned into grey, indicating aggregation.
This is clearly reflected by a well-defined red-shift of the absorption peak and significantly increased absorbance in 550–800 nm regimes.
This label-free low-cost assay should find applications in food analysis, genetic screening, and homology studies.
American Psychological Association (APA)
Ali, M. E.& Hashim, U.& Mustafa, Shuhaimi& Che Man, Y. B.& Islam, Kh. Nurul. 2011. Gold Nanoparticle Sensor for the Visual Detection of Pork Adulteration in Meatball Formulation. Journal of Nanomaterials،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-1029126
Modern Language Association (MLA)
Ali, M. E.…[et al.]. Gold Nanoparticle Sensor for the Visual Detection of Pork Adulteration in Meatball Formulation. Journal of Nanomaterials No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-1029126
American Medical Association (AMA)
Ali, M. E.& Hashim, U.& Mustafa, Shuhaimi& Che Man, Y. B.& Islam, Kh. Nurul. Gold Nanoparticle Sensor for the Visual Detection of Pork Adulteration in Meatball Formulation. Journal of Nanomaterials. 2011. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-1029126
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1029126