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Synthesis of a New Potential Conjugated TAT-Peptide-Chitosan Nanoparticles Carrier via Disulphide Linkage
Joint Authors
Nik Dzulkefli, Nik Nur Shamiha
Sahudin, Shariza
Katas, Haliza
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-06
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Chitosan and TAT peptide have been widely investigated as delivery systems for various biomolecules such as plasmid DNA, oligonucleotides, and siRNAs.
Conjugation of chitosan with TAT-peptide was therefore expected to produce a carrier with enhanced ability to facilitate cellular uptake.
In this study, chitosan nanoparticles (CNs) were prepared by ionic gelation method prior to conjugation with TAT-peptide via disulphide linkage (CN-TAT).
The conjugation was performed at various TAT-peptide-to-chitosan weight ratios ranging from 0.008 : 1 to 0.125 : 1.
siRNA as a model biomolecule was loaded by adsorption onto the CN-TAT.
Nanosize range particles were produced with a size range of less than 700 nm depending on TAT-peptide concentration used.
HPLC and Raman spectrometry analysis revealed that TAT-peptide was successfully conjugated to the CN via disulphide linkage.
siRNA loading efficiency for CN-TAT was 93% ±0.01.
In vitro cytotoxicity studies showed that CN-TAT has relatively low toxicity.
In conclusion, TAT conjugated onto CN via disulphide linkage was successfully synthesized, and its low cytoxicity demonstrates a potential for its use as a vector for biomolecules.
American Psychological Association (APA)
Katas, Haliza& Nik Dzulkefli, Nik Nur Shamiha& Sahudin, Shariza. 2012. Synthesis of a New Potential Conjugated TAT-Peptide-Chitosan Nanoparticles Carrier via Disulphide Linkage. Journal of Nanomaterials،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-1029136
Modern Language Association (MLA)
Katas, Haliza…[et al.]. Synthesis of a New Potential Conjugated TAT-Peptide-Chitosan Nanoparticles Carrier via Disulphide Linkage. Journal of Nanomaterials No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-1029136
American Medical Association (AMA)
Katas, Haliza& Nik Dzulkefli, Nik Nur Shamiha& Sahudin, Shariza. Synthesis of a New Potential Conjugated TAT-Peptide-Chitosan Nanoparticles Carrier via Disulphide Linkage. Journal of Nanomaterials. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-1029136
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1029136