Cytotoxicity of Silver Nanoparticles in Human Embryonic Stem Cell-Derived Fibroblasts and an L-929 Cell Line

Abstract EN

Consensus about the toxicity of silver nanoparticles (Ag-NPs) has not been reached, even though extensive attention has been paid to this issue.

This confusion may be due to physicochemical factors of Ag-NPs and the cell model used for biological safety evaluation.

In the present study, human embryonic stem cell-derived fibroblasts (EBFs), which have been considered a closer representative of the in vivo response, were used as a novel cell model to assess the cytotoxicity of Ag-NPs (~20 nm and ~100 nm) in comparison with L-929 fibroblast cell line.

Cell proliferation, cell cycle, apoptosis, p53 expression, and cellular uptake were examined.

Results showed that Ag-NPs presented higher cytotoxicity to EBF than to L-929.

EBF demonstrated a stronger capacity to ingest Ag-NPs, a higher G2/M arrest, and more upgraduated p53 expression after exposed to Ag-NPs for 48 h when compared with L-929.

It could be concluded that EBF exhibited a more sensitive response to Ag-NPs compared with L-929 cells, indicating that EBF may be a valid candidate for cytotoxicity screening assays of nanoparticles.