Synthesis of Antibodies-Conjugated Fluorescent Dye-Doped Silica Nanoparticles for a Rapid Single Step Detection of Campylobacter jejuni in Live Poultry
Joint Authors
Tansub, Wachira
Theparoonrat, Supannee
Limsuwan, P.
Tuitemwong, Kooranee
Tuitemwong, Pravate
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-08
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The preparation of antibodies-conjugated fluorescent dye-doped silica nanoparticles (FDS-NPs) was developed to detect Campylobacter jejuni cells under a fluorescence microscope.
The particles prepared by sol-gel microemulsion techniques have a round shape with an average size of 43 ± 4 nm.
They were highly photo stable and could emit strong orange fluorescent for 60 min.
Both amine- and carboxyl-functionalized properties were evident from FTIR and FT Raman spectra.
The FDS-NPs conjugated with antibodies against C.
jejuni were well dispersed in PBS solution at 20 mM of NaCl.
The conjugation with monoclonal antibodies against C.
jejuni was successful.
The direct observation of the antibodies-conjugated FDS-NPs- that bounds C.
jejuni with Petroff Hausser counting chamber at 40x was clear.
The different focus lengths clearly separated bound and unbound FDS-NPs under the microscope.
We successfully synthesis the bio-conjugated dye doped silica nanoparticles for C.
jejuni that are easy to use and giving clear detection in due time.
American Psychological Association (APA)
Tansub, Wachira& Tuitemwong, Kooranee& Limsuwan, P.& Theparoonrat, Supannee& Tuitemwong, Pravate. 2012. Synthesis of Antibodies-Conjugated Fluorescent Dye-Doped Silica Nanoparticles for a Rapid Single Step Detection of Campylobacter jejuni in Live Poultry. Journal of Nanomaterials،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-1029302
Modern Language Association (MLA)
Tansub, Wachira…[et al.]. Synthesis of Antibodies-Conjugated Fluorescent Dye-Doped Silica Nanoparticles for a Rapid Single Step Detection of Campylobacter jejuni in Live Poultry. Journal of Nanomaterials No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-1029302
American Medical Association (AMA)
Tansub, Wachira& Tuitemwong, Kooranee& Limsuwan, P.& Theparoonrat, Supannee& Tuitemwong, Pravate. Synthesis of Antibodies-Conjugated Fluorescent Dye-Doped Silica Nanoparticles for a Rapid Single Step Detection of Campylobacter jejuni in Live Poultry. Journal of Nanomaterials. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-1029302
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1029302