Assessment of Pulmonary Fibrogenic Potential of Multiwalled Carbon Nanotubes in Human Lung Cells
Joint Authors
Mercer, Robert R.
Castranova, Vincent
Mishra, Anurag
Chen, Bean T.
Wang, Liying
Rojanasakul, Yon
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-31
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Multiwalled carbon nanotubes have been shown to possess unusual fibrogenic activity in vivo and are currently the focus of intense toxicological investigations.
This study further determines the fibrogenic potential of well-dispersed MWCNT in human lung cell culture models and to develop a novel platform for understanding the cellular mechanisms of MWCNT-induced lung fibrosis.
Survanta, a natural lung surfactant, showed effectiveness in dispersing agglomerates of MWCNT to fine structures similar in size to aerosolized one.
At relevant low doses (0.002–0.2 μg/cm2), MWCNT exhibited a dose-dependent bio-effect on the human lung epithelial cells which is more pronounced in dispersed-MWCNT compared to non-dispersed form.
Significantly elevated levels of fibrogenic mediators, such as transforming growth factor-β1 and matrix metalloprotienases-9 were observed in the dispersed-MWCNT treated lung epithelial cells.
Based on previous in vivo studies showing that dispersed-MWCNT penetrated the interstitium and caused rapid interstitial fibrosis, we evaluated the potential direct interaction between lung fibroblasts and MWCNT.
Direct stimulation of human lung fibroblast cell proliferation, collagen expression and fibroblast growth factor-2 were observed which suggests novel mechanisms of MWCNT-induced lung fibrosis.
Our results indicate that the dispersion status of MWCNT determines their fibrogenic activity which is consistent with in vivo findings.
American Psychological Association (APA)
Mishra, Anurag& Rojanasakul, Yon& Chen, Bean T.& Castranova, Vincent& Mercer, Robert R.& Wang, Liying. 2012. Assessment of Pulmonary Fibrogenic Potential of Multiwalled Carbon Nanotubes in Human Lung Cells. Journal of Nanomaterials،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-1029322
Modern Language Association (MLA)
Mishra, Anurag…[et al.]. Assessment of Pulmonary Fibrogenic Potential of Multiwalled Carbon Nanotubes in Human Lung Cells. Journal of Nanomaterials No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-1029322
American Medical Association (AMA)
Mishra, Anurag& Rojanasakul, Yon& Chen, Bean T.& Castranova, Vincent& Mercer, Robert R.& Wang, Liying. Assessment of Pulmonary Fibrogenic Potential of Multiwalled Carbon Nanotubes in Human Lung Cells. Journal of Nanomaterials. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-1029322
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1029322