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Multispectroscopic Study of the Interaction of Chloramphenicol with Human Neuroglobin
Joint Authors
Gao, Chaohui
Huang, Lei
Li, Lianzhi
Li, Haili
Cui, Hui
Tan, Xiangshi
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-03
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The interaction between chloramphenicol (CHL) and neuroglobin (Ngb) has been investigated by using fluorescence, synchronous fluorescence, UV-Vis and circular dichroism (CD) spectroscopy.
It has been found that CHL molecule can quench the intrinsic fluorescence of Ngb in a way of dynamic quenching mechanism, which was supported by UV-Vis spectral data.
Their effective quenching constants (KSV) are 2.2×104, 2.6×104, and 3.1×104 L·mol−1 at 298 K, 303 K, and 308 K, respectively.
The enthalpy change (ΔH) and entropy change (ΔS) for this reaction are 26.42 kJ·mol−1 and 171.7 J·K−1, respectively.
It means that the hydrophobic interaction is the main intermolecular force of the interaction between CHL and Ngb.
Synchronous fluorescence spectra showed that the microenvironment of tryptophan and tyrosine residues of Ngb has been changed slightly.
The fluorescence quenching efficiency of CHL to tyrosine residues is a little bit more than that to tryptophan residues of Ngb.
Furthermore, CD spectra indicated that CHL can induce the formation of α-helix of Ngb.
American Psychological Association (APA)
Huang, Lei& Li, Lianzhi& Li, Haili& Gao, Chaohui& Cui, Hui& Tan, Xiangshi. 2012. Multispectroscopic Study of the Interaction of Chloramphenicol with Human Neuroglobin. Journal of Spectroscopy،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-1029350
Modern Language Association (MLA)
Huang, Lei…[et al.]. Multispectroscopic Study of the Interaction of Chloramphenicol with Human Neuroglobin. Journal of Spectroscopy No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-1029350
American Medical Association (AMA)
Huang, Lei& Li, Lianzhi& Li, Haili& Gao, Chaohui& Cui, Hui& Tan, Xiangshi. Multispectroscopic Study of the Interaction of Chloramphenicol with Human Neuroglobin. Journal of Spectroscopy. 2012. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-1029350
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1029350