Study of Cellular Uptake of Modified Oligonucleotides by Using Time-Resolved Microspectrofluorimetry and Florescence Imaging
Joint Authors
Kočišová, E.
Praus, P.
Štěpánek, Josef
Mojzeš, Peter
Sureau, F.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-11
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Fluorescence microimaging and homodyne phase-resolved confocal microspectrofluorimetry were used to monitor the transport of antisense oligonucleotide into cancer MCF7 cells and the subsequent intracellular distribution.
Phosphorothioate analog of 15-mer oligoadenylate (dA15) labeled by ATTO 425 was complexed with 5,10,15,20-tetrakis (1-methyl-4-pyridyl) porphyrin (H2TMPyP4) as an uptake-mediating agent.
Fluorescence lifetime data within a broad spectral range have revealed properties of both components inside the cell.
H2TMPyP4 lifetime inside the cell is not influenced in this malignant cell line, while the lifetime of modified oligonucleotide was found to be slightly shortened.
American Psychological Association (APA)
Praus, P.& Kočišová, E.& Mojzeš, Peter& Štěpánek, Josef& Sureau, F.. 2012. Study of Cellular Uptake of Modified Oligonucleotides by Using Time-Resolved Microspectrofluorimetry and Florescence Imaging. Journal of Spectroscopy،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-1029352
Modern Language Association (MLA)
Praus, P.…[et al.]. Study of Cellular Uptake of Modified Oligonucleotides by Using Time-Resolved Microspectrofluorimetry and Florescence Imaging. Journal of Spectroscopy No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-1029352
American Medical Association (AMA)
Praus, P.& Kočišová, E.& Mojzeš, Peter& Štěpánek, Josef& Sureau, F.. Study of Cellular Uptake of Modified Oligonucleotides by Using Time-Resolved Microspectrofluorimetry and Florescence Imaging. Journal of Spectroscopy. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-1029352
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1029352