Structural Perturbation of Superfolder GFP in the Presence of Guanidine Thiocyanate
Joint Authors
Stepanenko, Olga V.
Stepanenko, Olesya V.
Kuznetsova, Irina M.
Verkhusha, Vladyslav V.
Turoverov, Konstantin K.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-11
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The guanidine-thiocyanate-induced denaturation-renaturation of sfGFP was studied.
It was shown that the disruption of sfGFP native structure occurs in the range of guanidine thiocyanate concentrations from 0.5 to 2.5 M.
This process was accompanied by simultaneous changes of all recorded parameters.
It was found that the small guanidine thiocyanate concentrations (less then 0.1-0.2 M) triggered local structural disturbances in protein which result in significant decrease of chromophore and tryptophan fluorescence intensity and change of protein visible absorption spectrum.
American Psychological Association (APA)
Stepanenko, Olesya V.& Stepanenko, Olga V.& Kuznetsova, Irina M.& Verkhusha, Vladyslav V.& Turoverov, Konstantin K.. 2012. Structural Perturbation of Superfolder GFP in the Presence of Guanidine Thiocyanate. Journal of Spectroscopy،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-1029362
Modern Language Association (MLA)
Stepanenko, Olesya V.…[et al.]. Structural Perturbation of Superfolder GFP in the Presence of Guanidine Thiocyanate. Journal of Spectroscopy No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-1029362
American Medical Association (AMA)
Stepanenko, Olesya V.& Stepanenko, Olga V.& Kuznetsova, Irina M.& Verkhusha, Vladyslav V.& Turoverov, Konstantin K.. Structural Perturbation of Superfolder GFP in the Presence of Guanidine Thiocyanate. Journal of Spectroscopy. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-1029362
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1029362
