Effects of Trauma-Hemorrhage and IL-6 Deficiency on Splenic Immune Function in a Murine Trauma Model
Joint Authors
Mommsen, Philipp
Andruszkow, Hagen
Barkhausen, T.
Neunaber, C.
Krettek, Christian
Zeckey, Christian
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-09
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Splenic immune function is known to be depressed following hemorrhage.
The present study investigates the effects of femoral shaft fracture, isolated or in combination with hemorrhage, on early stage cytokine production capacity of splenocytes and observes the role of IL-6 under these conditions.
Male IL-6 knockout (IL-6−/−) and wild-type mice (WT) were randomly divided into three groups: sham (S), isolated femoral fracture (Fx), and femoral fracture + volume controlled hemorrhage (TH-Fx) (n=6 per group).
Animals were sacrificed four hours after induction of hemorrhage and fracture.
Cytokine release (TNF-α, IL-6, and IL-10) of isolated and LPS-stimulated splenocytes was determined by cytometric bead array.
Femoral fracture with or without hemorrhage caused a suppression of in vitro cytokine production capacity of splenocytes at an early posttraumatic stage in WT and IL-6−/−.
In the absence of IL-6, the profile of splenic cytokine secretion is significantly altered, identifying this cytokine as a potential therapeutic target to modulate the posttraumatic immune response.
American Psychological Association (APA)
Mommsen, Philipp& Barkhausen, T.& Zeckey, Christian& Andruszkow, Hagen& Krettek, Christian& Neunaber, C.. 2012. Effects of Trauma-Hemorrhage and IL-6 Deficiency on Splenic Immune Function in a Murine Trauma Model. Mediators of Inflammation،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-1029376
Modern Language Association (MLA)
Mommsen, Philipp…[et al.]. Effects of Trauma-Hemorrhage and IL-6 Deficiency on Splenic Immune Function in a Murine Trauma Model. Mediators of Inflammation No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-1029376
American Medical Association (AMA)
Mommsen, Philipp& Barkhausen, T.& Zeckey, Christian& Andruszkow, Hagen& Krettek, Christian& Neunaber, C.. Effects of Trauma-Hemorrhage and IL-6 Deficiency on Splenic Immune Function in a Murine Trauma Model. Mediators of Inflammation. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-1029376
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1029376