Human Mast Cells (HMC-1 5C6) Enhance Interleukin-6 Production by Quiescent and Lipopolysaccharide-Stimulated Human Coronary Artery Endothelial Cells
Joint Authors
Vineesh, V. R.
Walia, Damandeep S.
Sharma, Mukut
Zhou, Jianping
Sharma, Ram
Stechschulte, Daniel J.
Dileepan, Kottarappat N.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-01-23
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
We examined the effect of intact human mast cells (HMC-1 5C6) and their selected mediators on interleukin-6 (IL-6) production and bone morphogenetic protein-2 (BMP-2) expression in human coronary artery endothelial cells (HCAEC) in the presence and absence of lipopolysaccharide (LPS).
Scanning electron microscopy showed that HMC-1 5C6 cells adhere to HCAEC in cocultures.
Addition of HMC-1 5C6 cells markedly enhanced the IL-6 production by quiescent and LPS-activated HCAEC even at the maximal concentration of LPS.
Furthermore, mast cell-derived histamine and proteases accounted for the direct and synergistic effect of mast cells on IL-6 production that was completely blocked by the combination of histamine receptor-1 antagonist and protease inhibitors.
Another novel finding is that histamine was able to induce BMP-2 expression in HCAEC.
Collectively, our results suggest that endotoxin and mast cell products synergistically amplify vascular inflammation and that histamine participates in the early events of vascular calcification.
American Psychological Association (APA)
Walia, Damandeep S.& Sharma, Mukut& Vineesh, V. R.& Zhou, Jianping& Sharma, Ram& Stechschulte, Daniel J.…[et al.]. 2012. Human Mast Cells (HMC-1 5C6) Enhance Interleukin-6 Production by Quiescent and Lipopolysaccharide-Stimulated Human Coronary Artery Endothelial Cells. Mediators of Inflammation،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-1029386
Modern Language Association (MLA)
Walia, Damandeep S.…[et al.]. Human Mast Cells (HMC-1 5C6) Enhance Interleukin-6 Production by Quiescent and Lipopolysaccharide-Stimulated Human Coronary Artery Endothelial Cells. Mediators of Inflammation No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-1029386
American Medical Association (AMA)
Walia, Damandeep S.& Sharma, Mukut& Vineesh, V. R.& Zhou, Jianping& Sharma, Ram& Stechschulte, Daniel J.…[et al.]. Human Mast Cells (HMC-1 5C6) Enhance Interleukin-6 Production by Quiescent and Lipopolysaccharide-Stimulated Human Coronary Artery Endothelial Cells. Mediators of Inflammation. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-1029386
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1029386